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An Lgr5-independent developmental lineage is involved in mouse intestinal regeneration

ABSTRACT: An Lgr5-independent developmental lineage is involved in mouse intestinal regeneration

PROVIDER: PRJNA1092561 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28469932_1.fastq.gz	Fastqsanger.gz
	SRR28469932_2.fastq.gz	Fastqsanger.gz
	SRR28469933_1.fastq.gz	Fastqsanger.gz
	SRR28469933_2.fastq.gz	Fastqsanger.gz
	SRR28469934_1.fastq.gz	Fastqsanger.gz

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An Lgr5-independent developmental lineage is involved in mouse intestinal regeneration

Project description:Collagenase/dispase treatment of intestinal tissue from adult mice generates cells growing in matrigel as immortal cystic spheroids in addition to differentiated organoids. Contrary to classical EDTA-derived organoids, these spheroids display poor intestinal differentiation and are independent of Rspondin/Noggin/EGF for growth. Their transcriptome resembles strikingly that of fetal intestinal spheroids, with downregulation of crypt base columnar cell (CBC) markers (Lgr5, Ascl2, Smoc2, Olfm4). In addition, they display upregulation of inflammatory and mesenchymal genetic programs, together with robust expression of YAP target genes. Lineage tracing, cell-sorting and single cell RNA sequencing experiments demonstrate that adult spheroid-generating cells belong to a hitherto undescribed developmental lineage, independent of Lgr5+ve CBCs, and are involved in regeneration of the epithelium following CBC ablation

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An Lgr5-independent developmental lineage is involved in mouse intestinal regeneration II

Project description:Collagenase/dispase (CD fraction) treatment of intestinal tissue from adult mice generates cells growing in matrigel as immortal cystic spheroids in addition to differentiated organoids. Contrary to classical EDTA-derived organoids, these spheroids display poor intestinal differentiation and are independent of Rspondin/Noggin/EGF for growth. Their transcriptome resembles strikingly that of fetal intestinal spheroids, with downregulation of crypt base columnar cell (CBC) markers (Lgr5, Ascl2, Smoc2, Olfm4). In addition, they display upregulation of inflammatory and mesenchymal genetic programs, together with robust expression of YAP target genes. Lineage tracing, cell-sorting and single cell RNA sequencing experiments demonstrate that adult spheroid-generating cells belong to a hitherto undescribed developmental lineage, independent of Lgr5+ve CBCs, and are involved in regeneration of the epithelium following CBC ablation

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An Lgr5-independent developmental lineage is involved in mouse intestinal regeneration II

Project description:An Lgr5-independent developmental lineage is involved in mouse intestinal regeneration II

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An Lgr5-independent developmental lineage is involved in mouse intestinal regeneration (ATAC seq)

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An Lgr5-independent developmental lineage is involved in mouse intestinal regeneration [scRNA-Seq]

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Project description:An Lgr5-independent developmental lineage is involved in mouse intestinal regeneration [Organoids vs Spheroids in sandwich protocol]

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