Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

An immunohistochemical atlas of necroptotic pathway expression

ABSTRACT: An immunohistochemical atlas of necroptotic pathway expression

PROVIDER: PRJNA1093127 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR28491676_1.fastq.gz	Fastqsanger.gz
	SRR28491676_2.fastq.gz	Fastqsanger.gz
	SRR28491677_1.fastq.gz	Fastqsanger.gz
	SRR28491677_2.fastq.gz	Fastqsanger.gz
	SRR28491678_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

An immunohistochemical atlas of necroptotic pathway expression

Project description:Necroptosis is a lytic form of regulated cell death reported to contribute to inflammatory diseases of the gut, skin and lung, as well as ischemic-reperfusion injuries of the kidney, heart and brain. However, precise identification of the cells and tissues that undergo necroptotic cell death in vivo has proven challenging in the absence of robust protocols for immunohistochemical detection. Here, we provide automated immunohistochemistry protocols to detect core necroptosis regulators – Caspase-8, RIPK1, RIPK3 and MLKL – in formalin-fixed mouse and human tissues. We observed surprising heterogeneity in protein expression within tissues, whereby short-lived immune barrier cells were replete with necroptotic effectors, whereas long-lived cells lacked RIPK3 or MLKL expression. Local changes in the expression of necroptotic effectors occurred in response to insults such as inflammation, dysbiosis or immune challenge, consistent with necroptosis being dysregulated in disease contexts. These methods will facilitate the precise localisation and evaluation of necroptotic signaling in vivo.

2024-04-17 | GSE262762 | GEO

An immunohistochemical atlas of necroptotic pathway expression

Project description:Necroptosis is a lytic form of regulated cell death reported to contribute to inflammatory diseases of the gut, skin and lung, as well as ischemic-reperfusion injuries of the kidney, heart and brain. However, precise identification of the cells and tissues that undergo necroptotic cell death in vivo has proven challenging in the absence of robust protocols for immunohistochemical detection. Here, we provide automated immunohistochemistry protocols to detect core necroptosis regulators - Caspase-8, RIPK1, RIPK3 and MLKL - in formalin-fixed mouse and human tissues. We observed surprising heterogeneity in protein expression within tissues, whereby short-lived immune barrier cells were replete with necroptotic effectors, whereas long-lived cells lacked RIPK3 or MLKL expression. Local changes in the expression of necroptotic effectors occurred in response to insults such as inflammation, dysbiosis or immune challenge, consistent with necroptosis being dysregulated in disease contexts. These methods will facilitate the precise localisation and evaluation of necroptotic signaling in vivo.

| S-SCDT-10_1038-S44321-024-00074-6 | biostudies-other

Activin pathway gene expression atlas in rainbow trout

Project description:Activin pathway gene expression atlas in rainbow trout

| PRJNA931955 | ENA

Proteomic analysis of necroptotic extracellular vesicles

Project description:Necroptosis is a regulated but inflammatory form of cell death. We and others have previously reported that necroptotic cells release extracellular vesicles (EVs). We found that necroptotic EVs are loaded with proteins, including the key necroptosis executor factor, phosphorylated mixed lineage kinase domain-like (pMLKL). However, the exact necroptotic EVs proteins composition and impact have not been delineated yet. To characterize their content, EVs from necroptotic and untreated U937 cells were isolated by ultracentrifugation and analyzed by mass spectrometry. A total of 3337 proteins were identified, sharing high similarity with exosome proteome databases. Unsupervised hierarchical clustering of identified proteins distinguished between necroptotic and control EVs. A total of 353 proteins was significantly upregulated in the necroptotic EVs. Among these are MLKL and caspase-8, as validated by immunoblot. Components of ESCRTIII machinery and inflammatory signaling were found to be enriched in the necroptotic EVs, as well as yet unreported components of vesicles formation and transport and necroptosis signaling pathways. Moreover, we show that necroptotic EVs can be phagocytosed by macrophages to modulate cytokine and chemokine secretion. Finally, we reveal that necroptotic EVs contain tumor neoantigen and are enriched with proteins annotated as “antigen processing and presentation” biological process. In summary, our study reveals a new layer of regulation during the early stage of necroptosis by the secretion of specific EVs that influence their microenvironment and may instigate innate and adaptive immune responses. Future investigation will shed light on new players in the necroptosis signaling and its related EVs, and will uncover the functional tasks accomplished by the cargo of these necroptotic EVs.

2021-11-11 | PXD018258 | Pride

Selaginella expression atlas

Project description:Selaginella expression atlas

| PRJEB33869 | ENA

Immunohistochemical Expression of Inhibin Beta A (INHBA) in Colorectal Carcinoma

Project description:A retrospective observational study to evaluate immunohistochemical expression of Inhibin Beta A (INHBA) in 60 cases of Colorectal Carcinoma, and compare levels of its expression to different clinicopathological criteria.

| 13466 | ecrin-mdr-crc

Selaginella expression atlas

Project description:We created a gene expression atlas of major organs and under environmental perturbations (temperature and light) of the lycophyte Selaginella moellendorffii

2020-01-21 | E-MTAB-8217 | biostudies-arrayexpress

Expression atlas of chickpea during development

Project description:Expression atlas of chickpea during development

| PRJNA622231 | ENA

A Seminavis robusta gene expression atlas

Project description:A Seminavis robusta gene expression atlas

| PRJEB36275 | ENA

Soybean cell atlas gene expression profile

Project description:Soybean cell atlas gene expression profile

| PRJNA983388 | ENA