Project description:RNAseq of CD4 cells from 83 individuals with musculoskeletal complains (age 51y, range 19-86. male 22%) were collected at the Rheumatology Clinic during 2022-2023 . Cells were activated with anti-CD3 for 48 hours.
Project description:Investigation of transcriptome dynamics of Japanese cedar (Cryptomeria japonica) in winter (Dec. 22-23, 2011) and summer (July 30-31, 2012). We investigated seasonal and diurnal transcriptome dynamics of Japanese cedar (Cryptomeria japonica) by analyzing shoot samples collected at four-hour interval for two days in winter and summer, respectively. We first collected sequence data of expressed genes from shoots to designed microarray probes. Microarray analysis revealed the significant difference of transcripts between summer and winter, and the diurnal transcriptome dynamic in summer.Statistical analysis indicated that about 7.7 % of unique genes showed diurnal rhythms with more than two-fold of peak-to-trough amplitude in summer.
Project description:Environmental samples of dissected Botryllus schlosseri colonies collected on Avery Point, Connecticut, during spring 2023
Seawater samples collected at the marina on Avery Point, Connecticut, during spring 2023.
2024-11-06 | MSV000096313 | MassIVE
Project description:Klebsiella pneumoniae OXA-48 in Israel, 2020-2023
Project description:Small RNA sequencing of plasma samples collected from Polish Longwool ewes during high-fat diet-induced obesity and leptin resistance. Six ovariectomized ewes received a high-fat diet (OBESE group) and six received a standard diet (CTRL group). Plasma samples were collected at three timepoints: week 7 (T1), week 10 (T2) and week 14 (T3) of the dietary intervention. Libraries were prepared using the QIAseq miRNA Library Kit (QIAGEN) and sequenced on the AVITI platform (Element Biosciences) using 1x76 single-end reads. This study was supported by the National Science Center, Poland (Grant no. UMO-2023/51/B/NZ9/00651).
Project description:To examine the difference of the endometrial conditions in the cows between on summer and autumn seasons, gene expression profiles were compared. The expressions of 268 genes were significantly higher in the endometrium collected on summer than those on autumn, whereas 369 genes were lower (P<0.05 or lower). Transcripts of GP2 and NTS were more abundant in the endometrium of summer than those of autumn (P<0.05). In contrast, the mRNA expressions of CDH1 and HSPH1 were lower in the endometrium of summer seasons (P<0.1). Collectively, the different gene expression profiles may contribute to functional differences of endometrium between the summer and autumn seasons, and GP2 and NTS may have a relevance to endometrial deficiency that cause infertility of cows on summer seasons.
2015-02-19 | GSE66057 | GEO
Project description:Landscape Transcriptomics: Bumble Bee Field Collections Summer 2023
Project description:Environmental samples of dissected Botryllus schlosseri colonies collected on Avery Point, Connecticut, during spring 2023
Seawater samples collected at the marina on Avery Point, Connecticut, during spring 2023.