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ABSTRACT: Hy-Line Brown hens

PROVIDER: PRJNA1095718 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR28538050_1.fastq.gz	Fastqsanger.gz
	SRR28538050_2.fastq.gz	Fastqsanger.gz
	SRR28538051_1.fastq.gz	Fastqsanger.gz
	SRR28538051_2.fastq.gz	Fastqsanger.gz
	SRR28538052_1.fastq.gz	Fastqsanger.gz

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E-MTAB-8350

Project description:RNA-seq for splenic gene expression in response to Newcastle disease virus challenge in Hy-Line Brown chickens

2020-01-10 | E-MTAB-8350 | ExpressionAtlas

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mRNA-seq of the jejunum mucosa in LSL and LB laying hens following the variations of dietary calcium and phosphorus levels

Project description:Calcium (Ca) and phosphorus (P) are essential micronutrients that are linked to a wide set of biological processes. In laying hens there is still uncertainty about the optimal Ca/P ratio in feed and further strategies for the reliable mineral restriction in poultry diets are required. The dataset is based on Lohmann Selected Leghorn (LSL) and Lohmann Brown (LB) laying hens sampled at peak performance. The experimental design comprises four dietary groups differed in Ca (recommended vs. 15 % reduction) and mineral P (adequate vs. 20 % reduction) levels; 1) control diet (Con; Ca=34.4g/kg, P=5.3 g/kg and Ca/P ratio=7.45), 2) Low Ca and P diet (LCaP), 3) low Ca diet (LCa), and low P diet (LP). Jejunal transcriptome profiles were assessed by mRNA sequencing in a total of 80 animals (10 hens per experimental group for each laying line) at sampling age of 31 weeks) to approximate the consequences of variable Ca and P supply.

2021-06-14 | E-MTAB-9109 | biostudies-arrayexpress

RNA-seq for splenic gene expression in response to Newcastle disease virus challenge in Hy-Line Brown chickens

Project description:In order to have a better understanding about genetic regulation of response to NDV challenge, and also to identify the candidate genes regulating disease resistance in combination with GWAS, gene expression in spleen of Hy-Line Brown chickens was investigated. The chickens were divided into two groups (8 chickens/group) that are respectively treated by NDV(200 ul of 108 EID50%) and Phosphate-buffered saline (PBS) through nasal and ocular inoculation routes at 21 days post hatch. Gene expression in spleen was then detected by RNA-seq at 2 and 6 day post inoculation (dpi).

2019-09-30 | E-MTAB-8350 | biostudies-arrayexpress

A survey of gene expression in adult chicken tissues using a chicken 20K oligonucleotide microarray

Project description:Transcriptional profiling of eight normal adult chicken tissues in 10-week old brown (lohmann brown) hens, the eight tissues include brain, bursa of Fabricius, jejunum, kidney, liver, lung, spleen, thymus. Keywords: normal chicken tissues, transcriptional profiling. eight different normal chicken adult tissue types, five biological replicates per tissue, each individual sample was hybridized with a common reference pool (pool of RNA samples from all individual samples). All individual samples were labeled with Cy3, common reference was labeled with Cy5.

2010-09-09 | E-GEOD-17108 | biostudies-arrayexpress

microRNA-seq profiling of the jejunum mucosa of two laying hen strains fed different dietary Ca and P levels at the egg production peak of 31 weeks of age

Project description:Calcium (Ca) and phosphorus (P) are essential micronutrients linked to arrays of biological processes and physiological conditions. In laying hens, the optimal Ca/P ratio in feed is inconsistent but necessary for reliable schemes of mineral restriction in poultry diets. This study investigates the effects of dietary treatments varying in the Ca and P levels in two laying hen strains (Lohmann Brown-Classic and Lohmann LSL-Classic) at the peak of egg production (31 weeks of age). Four dietary treatment groups were differed in Ca (recommended vs. 15 % reduction) and mineral P (adequate vs. 20 % reduction) levels; 1) control diet (Con; Ca=34.4g/kg, P=5.3 g/kg and Ca/P ratio=7.45), 2) Low Ca and P diet (LCaP), 3) low Ca diet (LCa), and 4) low P diet (LP). microRNA expression of the jejunum mucosa were profiled by microRNA sequencing in a total of 80 animals (10 hens per experimental diet group for each of the two laying line) at sampling age of 31 weeks. RNA-seq data of matched samples are also available (E-MTAB-9109).

2022-01-25 | E-MTAB-9136 | biostudies-arrayexpress

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