Project description:Hemozoin phagocytosis results in immunomodulation. In the present study, gene expression profiles were analyzed to identify transcriptional changes associated with two of the individual components of Hz in a model macrophage cell line. LPS-stimulated RAW 264.7 cells were exposed to the synthetic form of Hz, β-hematin (BH, 0.1 mg/mL) and HNE (35 μM) for 6 or 24 h. Keywords: variable treatment
Project description:Hsd17b4 is a very important for beta oxidation. However, influence of hsd17b4 on gene expression in RAW 264.7 cells is unknown yet. In this study, the influence of hsd17b4 knockout on gene expression in RAW 264.7 cells was investigated.
Project description:All samples were gathered from mouse RAW 264.7 cells (macrophages). Control total RNA was extracted from untreated RAW 264.7 cells cultured for 48 hours. Test total RNA was extracted from lipopolysaccharide (100ng/ml) and lipopolysaccharide-binding protein (100pM) treated RAW 264.4 cells cultured for 48 hours. Experiment design included three control vs test arrays and three dye swap arrays. Keywords: other
Project description:All samples were gathered from mouse RAW 264.7 cells (macrophages). Control total RNA was extracted from untreated RAW 264.7 cells cultured for 16 hours. Test total RNA was extracted from lipopolysaccharide (100ng/ml) and lipopolysaccharide-binding protein (100pM) treated RAW 264.4 cells cultured for 16 hours. Experiment design included three control vs test arrays and three dye swap arrays. Keywords: other
Project description:All samples were gathered from mouse RAW 264.7 cells (macrophages). Control total RNA was extracted from untreated RAW 264.7 cells cultured for 8 hours. Test total RNA was extracted from lipopolysaccharide (100ng/ml) and lipopolysaccharide-binding protein (100pM) treated RAW 264.4 cells cultured for 8 hours. Experiment design included three control vs test arrays and three dye swap arrays. Keywords: other
Project description:All samples were gathered from mouse RAW 264.7 cells (macrophages). Control total RNA was extracted from untreated RAW 264.7 cells cultured for 4 hours. Test total RNA was extracted from lipopolysaccharide (100ng/ml) and lipopolysaccharide-binding protein (100pM) treated RAW 264.4 cells cultured for 4 hours. Experiment design included three control vs test arrays and three dye swap arrays. Keywords: other
Project description:All samples were gathered from mouse RAW 264.7 cells (macrophages). Control total RNA was extracted from untreated RAW 264.7 cells cultured for 2 hours. Test total RNA was extracted from lipopolysaccharide (100ng/ml) and lipopolysaccharide-binding protein (100pM) treated RAW 264.4 cells cultured for 2 hours. Experiment design included three control vs test arrays and three dye swap arrays. Keywords: other
Project description:All samples were gathered from mouse RAW 264.7 cells (macrophages). Control total RNA was extracted from untreated RAW 264.7 cells cultured for 1 hour. Test total RNA was extracted from lipopolysaccharide (100ng/ml) and lipopolysaccharide-binding protein (100pM) treated RAW 264.4 cells cultured for 1 hour. Experiment design included three control vs test arrays and three dye swap arrays. Keywords: other
