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Global m6A-modified mRNAs in undecidualized and decidualized primary human endometrial stromal cells [meRIP-seq]

ABSTRACT: Global m6A-modified mRNAs in undecidualized and decidualized primary human endometrial stromal cells [meRIP-seq]

PROVIDER: PRJNA1096724 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28571951_1.fastq.gz	Fastqsanger.gz
	SRR28571951_2.fastq.gz	Fastqsanger.gz
	SRR28571952_1.fastq.gz	Fastqsanger.gz
	SRR28571952_2.fastq.gz	Fastqsanger.gz
	SRR28571953_1.fastq.gz	Fastqsanger.gz

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Global m6A-modified mRNAs in undecidualized and decidualized primary human endometrial stromal cells [meRIP-seq]

Project description:Global m6A-modified mRNAs in undecidualized and decidualized primary human endometrial stromal cells were analyzed by using methylated RNA immunoprecipitation sequencing (MeRIP-seq)

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Global m6A-modified mRNAs in mouse uterus on gestational day 4 [meRIP-seq]

Project description:Global m6A-modified mRNAs in wild-type mouse uterus on gestational day 4 were analyzed by using methylated RNA immunoprecipitation sequencing (MeRIP-seq)

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Gene expression profiles of decidualized human endometrial stromal cells knockdown for HOXA10

Project description:The homeobox gene HOXA10 plays a key role in endometrial differentiation during embryogenesis and is abundantly expressed in the adult endometrium and decidual cells. In the adult endometrium the expression of HOXA10 is regulated by estrogen and progesterone and the levels are highest in the decidualized cells. We have shown that HOXA10 is required in the endometrial cells to maintain the decidual cell phenotype. In this study we aimed to determine the molecular mechanisms by which HOXA10 maintains the decidual phenotype and the other roles it might have in decidual physiology. In vitro decidualized human endometrial stromal cells were knocked down for HOXA10 using siRNA and gene expression profiles of the scrambled and HOXA10 siRNA transfected cells were compared at 24, 48 and 72 h post transfection. Several genes having multiple functions were found to be differentially expressed. We postulate that HOXA10 is required by the decidual cells to support immune cell differentiation, trophoblast invasion and cellular remodeling. In vitro decidualized priamry cultures of human endometrial strmal cells transfected with a siRNA againts HOXA10 or a scrambled siRNA. Cells harvested at 24, 48 and 72 h post transfection. Dual channel hybridization with dye swap design in biological replicates

2012-02-14 | E-GEOD-35801 | biostudies-arrayexpress

miRNA expression profiles in decidualized and non-decidualized normal endometrial stromal cells (NESCs).

Project description:To identify the miRNAs which regulate endometrial decidualization, we explored differentially expressed miRNAs between untreated NESCs (n=4) and decidualized NESCs (n=4) using miRNA microarray. The miRNA expression profiles of untreated NESCs and decidualized NESCs were obtained, then the miRNAs were filtered by signal intensity (50.0<, at least one out of 8 samples have values within range) and flags (at least 13% of cases have good flags). We established the criteria for regulated genes: ratio ≥2.0-fold for up-regulated genes and ratio ≤0.5 for down-regulated genes.

2016-10-12 | GSE75424 | GEO

mRNA expression profiles in decidualized and non-decidualized normal endometrial stromal cells (NESCs).

Project description:To identify the mRNAs which regulate endomrtial decidualization, we explored differentially expressed mRNAs between untreated NESCs (n=4) and decidualized NESCs (n=4) using gene expression microarray. The mRNA expression profiles of untreated in NESCs and decidualized NESCs were obtained, then the mRNAs were filtered by signal intensity (50.0<, at least one out of 8 samples have values within range) and flags (at least 43% of cases have good flags). We established the criteria for regulated genes: Z-score ≥2.0 and ratio ≥2.0-fold for up-regulated genes, and Z-score ≤–2.0 and ratio ≤0.5 for down-regulated genes.

2016-10-12 | GSE75425 | GEO

Global m6A-modified mRNAs in the uterus of wild-type mouse

Project description:Global m6A-modified mRNAs in the uterus of wild-type mouse were analyzed by using methylated RNA immunoprecipitation sequencing (MeRIP-seq)

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Global m6A-modified mRNAs in mouse uterus on gestational day 4 [meRIP-seq]

Project description:Global m6A-modified mRNAs in mouse uterus on gestational day 4 [meRIP-seq]

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Gene expression profiles of decidualized human endometrial stromal cells knockdown for HOXA10

2012-02-14 | GSE35801 | GEO

Expression data from non-decidualized and decidualized endometrial stromal cell (ESCs) with or without WT1 knockdown

Project description:Expression data from non-decidualized and decidualized endometrial stromal cell (ESCs) with or without WT1 knockdown

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mRNA expression profiles in decidualized and non-decidualized endometriotic cyst stromal cells (ECSCs).

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2016-10-12 | GSE75423 | GEO

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