Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1097214 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR28578120_1.fastq.gz | Fastqsanger.gz | |||
| SRR28578120_2.fastq.gz | Fastqsanger.gz | |||
| SRR28578121_1.fastq.gz | Fastqsanger.gz | |||
| SRR28578121_2.fastq.gz | Fastqsanger.gz | |||
| SRR28578122_1.fastq.gz | Fastqsanger.gz |
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Project description:Purpose: The goal of this study are to reveal the internal mechanism of silicon increased Glycyrrhiza uralensis Fisch. seedlings drought-tolerance,salt-tolerance and salt-drought tolerance by RNA-Seq. Methods: mRNA profiles of Glycyrrhiza uralensis Fisch. Seedling in eight treatment: control treatment with or without silicon,salt treatment with or without silicon,drought treatment with or without silicon,salt-drought treatment with or without silicon. Each treatment group sequenced the aerial (stems, leaves, buds and all the above ground parts) and underground parts (roots and all the underground parts) respectively Results:A total of 48 samples were sequenced and 372.37GB of clean data was acquired. The clean data of every sample reached 5.96GB, and the percentage of Q30 base was 94.63% or above. Clean reads of each sample were sequentially aligned with the specified reference genome, and alignment efficiency ranged from 85.27% to 92.66%. Therefore, the transcriptome data of samples were obtained with a high correct rate and good genomic coverage.Results of sequencing analysis showed that compared with CK group, there were 1426 DEGs (771 up-regulated and 655 down-regulated), 1386 DEGs (571 up-regulated and 815 down-regulated) and 4192 DEGs (1668 up-regulated and 2524 down-regulated) in aerial part, and 1462 DEGs (730 up-regulated and 732 down-regulated), 2212 DEGs (939 up-regulated and 1273 down-regulated) and 3735 DEGs (1768 up-regulated and 1986 down-regulated) in underground part of D, S and SD group respectively. Conclusions: Our study help to better understand the underlying molecular mechanisms of silicon improve the drought-tolerance,salt-tolerance and salt-drought tolerance of G. uralensis.
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Project description:When aboveground parts of intact plants are exposed to volatile organic compounds emitted from neighboring con-/heterospecific plants that are artificially damaged or damaged by herbivores, the resistant responses are induced in the plants. Differential responses of plants to enantiomers of the same volatile compound have also been reported in Arabidopsis: the root became shorter when Arabidopsis seedlings are exposed to aerial borneol, and the dose-dependent root length reduction was significantly different between (+)- and (-)-borneol. We identified (+)-borneol dependent inductive genes in Arabidpsis in this transcriptome analysis.
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Project description:Placenta transcriptome from two species of Spalax. Associated with the article "Genome-wide adaptive complexes to underground stresses in blind mole rats Spalax."
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Project description:Contrary to the relative wealth of information regarding pathogen defense responses in aboveground plant parts, little is known about the mechanistic basis and regulation of plant immunity in root tissues. Aiming to further our fundamental understanding of root immune responses, we have investigated the interaction between rice and one of its major root pathogens, the oomycete Pythium graminicola. The specificic objectives of this study were twofold: i) to disentangle the molecular and genetic basis of the rice-Pythium interaction by comparing the transcriptome of rice roots at different times after inoculation with a highly virulent Pythium strains, and ii) to offer fundamental insights into the genetic architecture and regulation of rice disease resistance pathways operative in root tissue and to identify the molecular players controlling the possible nodes of convergence between these resistance conduits Comparison between P. graminicola- and mock-infected rice roots. Two treatments (infected and non-infected) x three timepoints (1, 2 and 4 days post inoculation) x three biological replicates
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