Control of epigenomic landscape and development of fetal male germ cells through L-serine metabolism
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1097648 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR28592023.fastq.gz | Fastqsanger.gz | |||
| SRR28592024.fastq.gz | Fastqsanger.gz | |||
| SRR28592025.fastq.gz | Fastqsanger.gz | |||
| SRR28592026.fastq.gz | Fastqsanger.gz |
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Project description:The proper development of fetal germ cells (FGCs) is vital for genetic and epigenetic information transmission in generations. We have depicted the transcriptional landscape of distinct phases FGCs and the interaction between FGCs and gonadal niche cells. However, the epigenetic dynamics of sequential phases FGCs still remains in dimness. Here applied with single cell sequencing techniques (scBS-seq and scCOO-seq), we analyzed the genome-wide DNA methylation and chromatin accessibility map of female mitosis, meiotic prophase, and oogenesis FGCs, as well as male mitosis and mitotic-arrest FGCs.
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Project description:To better understand the underlying dynamics driving formation of the spermatogonial stem cell (SSC) pool during development, we performed single-cell RNA-sequencing on mouse germ cells isolated from testes during late fetal and early postnatal development. Transcriptome profiling revealed that a subset of prospermatogonia during late fetal development becomes preprogrammed to adopt the SSC fate in postnatal life. Further computational analysis mapped the transcriptional dynamics of the SSC trajectory through developmental time to identify novel pathways and regulators involved in formation of the foundational SSC pool. Paired with in vivo gene expression analysis via a multi-transgenic mouse model and functional assessment of germ cell fate via transplantation, the findings of our study present a model for which stem cell fate is preprogrammed in the spermatogenic lineage.
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