Project description:Oilseed rape (Brassica napus, B. napus) is one of the most important oil crops globally, contributing significantly to the world's supply of vegetable oil. However, its production is severely threatened by Sclerotinia stem rot, a disease caused by the broad-host-range fungus Sclerotinia sclerotiorum (Lib.) de Bary (S. sclerotiorum). We have investigated the gene expression of J9712 and W40-OE2 during different time periods of Sclerotinia sclerotiorum infection through RNA-Seq analysis.
Project description:Global transcriptome profiling of suceptible and tolerant lines of Brassica napus infected with Sclerotinia sclerotiorum using a petal inoculation method that mimics field conditions.
Project description:Brassica napus leaves(18 days old) were inoculated by Sclerotinia sclerotiorum with leaves harvested after 12, 24 and 48 h. Arabidopsis thaliana full-genome 70mer microarray representing at least 23,686 genes were used.
Project description:This SuperSeries is composed of the following subset Series: GSE15337: Gene expression profiling soybean stem tissue early response to Sclerotinia sclerotiorum 1 GSE15338: Gene expression profiling soybean stem tissue early response to Sclerotinia sclerotiorum 3 GSE15339: Gene expression profiling soybean stem tissue early response to Sclerotinia sclerotiorum 4 GSE15340: Gene expression profiling soybean stem tissue early response to Sclerotinia sclerotiorum 2 Refer to individual Series
Project description:This SuperSeries is composed of the following subset Series: GSE13254: Sclerotinia infected vs Mock infected controls in B. napus (Westar) GSE13256: Zhong You 821 Sclerotinia infected vs Mock infected controls in B. napus (Zhong You 821) The fungal pathogen Sclerotinia sclerotiorum infects a broad range of dicotyledonous plant species and is the causative agent of stem rot in Brassica napus. To elucidate the mechanisms underlying the defense response, we studied the patterns of gene expression in a partially resistant variety of ZhongYou 821 (ZY821) and a susceptible line from Westar over five time points, 6, 12, 24, 48, and 72 hours post-inoculation (hpi) using a B. napus oligonucleotide microarray. For each cultivar, a two-dye experiment was run comparing infected to mock-infected stem tissue. For each time point, 6 microarray slides were done (3 biological replicates, with a dye swap for each biological replicate). Refer to individual Series
Project description:Sclerotinia sclerotiorum is a broad-host range necrotrophic pathogen which is the causative agent of Sclerotinia stem rot (SSR), and a major disease of soybean (Glycine max). A time course transcriptomic analysis was performed in both compatible and incompatible soybean lines to identify pathogenicity and developmental factors utilized by S. sclerotiorum to achieve pathogenic success.
Project description:This study provides a first large-scale cloning and characterization of Sclerotinia sclerotiorum milRNAs and milRNAs candidates. Two microRNA-like RNAs (milRNAs) and 42 milRNA candidates were identified by sequence analysis. These milRNAs and candidates provide new insights into the functional roles of small RNAs and adds new resources for the study of plant pathogenic fungi. We constructed a small RNA library from Sclerotinia sclerotiorum.
