Project description:Through high-throughput sequencing technology, we explored the regulatory genes related to the biofilm formation and biocontrol potential of strain NCD-2, which laid a foundation for further understanding the molecular interaction between cotton root exudates and the strain.
Project description:Biofilm formation is an important mechanism of survival and persistence for many bacterial pathogens. These multicellular communities contain subpopulations of cells that display vast metabolic and transcriptional diversity along with high recalcitrance to antibiotics and host immune defenses. Investigating the complex heterogeneity within biofilm has been hindered by the lack of a sensitive and high-throughput method to assess stochastic transcriptional activity and regulation between bacterial subpopulations, which requires single-cell resolution. We have developed an optimized bacterial single-cell RNA sequencing method, BaSSSh-seq, to study Staphylococcus aureus diversity during biofilm growth and transcriptional adaptations following immune cell exposure.
2024-11-04 | GSE270986 | GEO
Project description:High-throughput sequencing data of biofilm and floc sludge.
| PRJNA1132799 | ENA
Project description:High-throughput sequencing data of biofilm with and without spermidine
Project description:Transcription factor binding locations by ChIP followed by high throughput sequencing. To build and validate an automated Chromatin Immunoprecipitation and high throughput Illumina sequencing pipeline
