Project description:This study provides a clear and accurate dynamic transcriptome profile of mRNAs in rumen, reticulum, omasum and abomasum of yaks. The results include high-quality genomic data and help to elucidate the important roles of these mRNAs in regulation of growth, development and metabolism in yaks, and to further understand the molecular mechanisms underlying metabolic regulation of yak stomach tissues. At the same time, it provided a theoretical basis for age-appropriate weaning and supplementary feeding in yaks.
Project description:We aimed to elucidate the effects of feeding condition (indoor grain-feeding vs. grazing on pasture) on c-miRNAs in Japanese Black (JB) cattle (Wagyu). The cattle at 18 months old were divided into pasture feeding and conventional indoor grain feeding for 5 months. Microarray analysis of c-miRNAs from the plasma extracellular vesicles led to the detection of a total of 202 bovine miRNAs in the plasma, including 15 miRNAs that differed between the feeding conditions.
Project description:The yak (Bos grunniens) is a critical livestock breed in the plateau region, and changing the feeding system can significantly improve the growth performance of yaks. In this study, the effects of different feeding regimes on growth performance and the meat quality of yaks were comprehensively compared. The transcriptome and proteome of longissimus dorsi muscle were studied by RNA-seq and TMT techniques. Our study found that indoor feeding significantly improved the yak growth performance (such as the average daily gain and net meat) and meat quality level compared with traditional grazing feeding. In the grazing (Group G) vs. stabling groups (Group HF) yak comparison, 40 DEGs/DAPs showed the same mRNA and protein expression trends. These genes are associated with collagen binding, lipoxygenase pathway, and arachidonic acid metabolic process.PRM verified the reliability of the TMT results. Moreover, some pathways such as the "AMPK signaling pathway," "FoxO signaling pathway," "PPAR signaling pathway," and "Fatty acid metabolism" were significantly enriched in this study. These results expand our knowledge of meat quality in yak and provide practical information and more evidence for further insight into the biological mechanisms of meat quality traits.
2022-10-11 | PXD037303 |
Project description:Grazing yaks sequencing with different shrub coverage
| PRJNA622912 | ENA
Project description:Rumen microbiota of grazing yaks changes with growth stages
| PRJNA566442 | ENA
Project description:Differential Faecal Microbiome in Yaks Fed on Grazing Land and Confined Feed
Project description:Trypanosoma (Megatrypanum) theileri is a ubiquitous parasite of Bovinae (cattle, buffalo, yaks and some antelopes). Here were report the transcriptome sequence of this parasite
Project description:By comparing transcriptomes of tolerant and intolerant plants of sickle alfalfa subject to intensive animal grazing, we identified pathways involved in nutrient-responsive signaling, light and wound response, cell wall formation, and energy metabolism. In these pathways, grazing suppressed 39 genes, but less severe in the tolerant plant, and activated 5 genes all carrying polymorphisms in their homologous transcripts between the tolerant and intolerant plants. These genes and pathways - responsive to grazing and differentially expressed between the tolerant and intolerant plants â?? underline a defense mechanism in alfalfa against grazing stresses. We examined transcriptomes of 3 alfalfa plants: grazing tolerant and grazed, grazing tolerant and not grazed, grazing intolerant and grazed, all at single replicate each.
Project description:To investigate the potential effect of grazing movement on miRNA circulation in cattle, here we profiled miRNA expression in centrifugally prepared exosomes from the plasma of both grazing and housed Japanese Shorthorn cattle. Microarray analysis of the c-miRNAs resulted in detection of a total of 231 bovine exosomal miRNAs in the plasma, with a constant expression level of let-7g across the duration and cattle groups. Expression of muscle-specific miRNAs such as miR-1, miR-133a, miR-206, miR-208a/b, and miR-499 were undetectable, suggesting the mildness of grazing movement as exercise.
Project description:Purpose:Yak long-term colonization and widespread distribution across the plateau can be serve as an ideal natural animal model to provide insights into the adaptive evolution of other plateau species, including humans. Methods:To exploring the molecular mechanisms of lung tissue in yak to response to hypoxia, the mRNA, lncRNA and miRNA of lung tissue from cattle and three different altitude yaks were sequenced. Results:A total of 21764 mRNAs, 14168 lncRNAs and 1209miRNAs (305 known and 904 novel miRNAs)were identifed.Compared yak with cattle, 4975 mRNAs, 3326 lncRNAs and 75 miRNAs were differentially expressed. 756 mRNAs, 346 lncRNAs and 83 miRNAs were found to be differentially expressed amongthree different altitude yaks(fold change≥2 and P-value<0.05). Conclusions:The differentially expressed genes were functionally enriched in long-chain fatty acid metabolic process and protein processing between yak and cattle, while the immune response and cell cycle were enriched among three different altitude yaks. Furthermore, the competing endogenous RNAs (ceRNAs) networks were identified to illustrate their roles.