Project description:Resistant lines (Mandelup, carrying the AnMan allele and 83A:476 carrying the Lanr1 allele), the line which is putative novel donor of resistance (Boregine) and a susceptible line (Population 22660) were used in this study. 4 weeks after sowing, when the plants reached 4-6 leaf stage, plants were inoculated with Colletotrichum lupini or mock-inoculated (control plants) and then kept for 24 hours in darkness under ~100% humidity and temperature of 25°C. Leaves were sampled at 6, 12, 24, 36, and 48 hours post inoculation.
