Project description:Dendritic cells from three healthy human donors were cultured in the presence or absence of either Aspergillus fumigatus, Saccharomyces cerevisiae, Candida albicans or Candida parapsilosis. Each of the four fungi were also cultured in the absence of human cells. RNA-sequencing was used to evaluate differences in the transcriptomes of human cells challenged and unchallenged with each fungal pathogen, as well as in those of each fungus challenged and unchallenged by cells from the human immune system.
Project description:Here, we develop a systems-level approach leveraging powerful next generation sequencing, proteomics and phenotypic studies to rapidly obtain an integrated view of lignocellulose degradation in the earliest free living fungi
Project description:Here, we develop a systems-level approach leveraging powerful next generation sequencing, proteomics and phenotypic studies to rapidly obtain an integrated view of lignocellulose degradation in the earliest free living fungi RNA-seq of Piromyces grown on Glucose, Cellulose, Cellulobiose, Avicel, Filter paper, and time-course of transient glucose pulse (catabolite repression). N>=2
Project description:To determine the mechanisms of improved toxic metal tolerance of A. arenosa inoculated with Mucor sp. whole gemone microarray profiling was employed. Metal toxicity altered the expression of 5169 probe sets; 2861 genes were up-regulated and 2308 down-regulated. Inoculation with the fungi induced changes in the expression of 899 probes from the above presented data set; 492 genes were up-regulated and 314 were down-regulated. The expression of 93 genes (63 and 30, up and down-regulated respectively) was affected by both treatments (Fig. 5A-B). Genes differentially expressed in E+ plants vs E- from mine dump substrate were subject to GO analysis (gene ontology) to identify enriched terms with a cut-off rate of 0.01. The majority of the genes upregulated belonged to ROS metabolism, response to biotic and abiotic stimuli, response to ion starvation and ET biosynthesis and signaling functional categories. Among down-regulated transcripts GO analysis revealed a significant enrichment in the regulation of anthocyanin biosynthesis term.
Project description:ChIP-seq on human vagina For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf
Project description:miRNA-seq on human vagina For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf
Project description:miRNA-seq on human vagina For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf
Project description:snATAC-seq of human vagina For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf
