Project description:Microarray analysis was used to identify candidate genes involved in the formation of hydroxycinnamoyl-arabinoxylan (AX) and secondary cell walls in rice. Gene expression in the internode pith parenchyma of Fukei71 (F71), the rice dwarf mutant that accumulates large amounts of hydroxycinnamoyl-AX in its pith parenchyma cells, was compared with the gene expression in wild-type pith parenchyma cells. Gene expression in wild-type whole internodes containing cells with thickened secondary cell walls, such as vascular and cortical fiber cells, was compared to the gene expression in wild-type internode pith parenchyma cells without any secondary cell walls.
Project description:Inbred mouse strains C57BL/6J (B6) and C3H/HeJ (C3H) exhibit a marked difference in their susceptibility to atherosclerosis and the arterial wall has proven to be a source of the difference in atherosclerosis susceptibility. Genome-wide gene expression analysis was conducted in aortic walls of the two strains. Total RNA was extracted from aortas of 6-week-old female B6 and C3H apoE-deficient (apoE-/-) mice fed a chow or Western diet. 1514 genes in chow fed mice and 590 genes in Western fed mice were found to be differentially expressed between the two strains. RNA was extracted from aorta using a Trizol protocol. Total RNA was pooled in an equal amount from 4 mice for each group. Standard Affymetrix procedures were performed using 8ug of total RNA. Microarrays were used to detect gene expression in aortic walls of two apoE-deficient mouse strains when fed a chow or western diet. Keywords: atherosclerosis, arterial walls, C57BL/6, C3H/HeJ, Inbred strains, Hyperlipidemia, and Western diet