Project description:We report the application of Illumina sequencing for high-throughput profiling of miRNA in citrus root responded to long-term boron toxicity. We find miR319 is involved in citrus adapation to long-term boron toxicity via targeting a MYB gene, Ciclev10000756m.g.v1.0, which is homologus with several MYBs that modulate lateral root development in Arabidopsis.
2021-02-25 | GSE111128 | GEO
Project description:Full-length transcriptome analysis of maize root tips under cold stress
Project description:After long-term different boron and aluminum treatment, 2D electrophoresis and mass spectrum were conducted to investigate different proteiomic profile in Citrus sinensis roots samples.
Project description:After long-term different boron and aluminum treatment, 2D electrophoresis and mass spectrum were conducted to investigate different proteiomic profile in Citrus grandis roots samples.
Project description:Boron is essential for plants, and boron availability in soil is an important determinant of agricultural production. Boron availability in soil is limited at many regions in the world, including Japan. Under boron deficient conditions, leaf expansion and root elongation, apical dominance, flower development,and fruit and seed sets are inhibited. In this work, we analyzed the mRNA expression of genes containing AUGUAA motif in their 5M-bM-^@M-2-UTR, which is induced by boron. We used microarrays to detail the global gene expression underlying boron deficiency in roots. Plants were grown on solid medium containing 1% (w/v) sucrose, 1.5% (w/v) gellan gum and 100 M-BM-5M boron for 10 days and then transferred to 0.3 and 100 M-BM-5M boron for 2 days. Plates were placed vertically at 22M-BM-0C in a growth chamber under long-day conditions (16 h light/8 h dark cycle). We analyzed the transcript profiles in roots by microarray analysis (Affymetrix ATH1 Genome Array).
Project description:To better understand the transcriptome of Nucleus Accumbens (NAc) – a key brain reward region – under chronic cocaine treatment, we perform the first Iso-Seq analysis on its mRNAs to determine the full-length transcripts without assembly.
