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ABSTRACT:
PROVIDER: PRJNA1101667 | ENA |
REPOSITORIES: ENA
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Project description:Hydrostatic pressure is one of the physical factors affecting cellular physiology. Hydrostatic pressure of a few hundred MPa decreases the viability of yeast cells, and pressure of a few tens MPa decreases the growth rate. To understand the effect of hydrostatic pressure, we employed yeast, Saccharomyces cerevisiae, DNA microarrays and analyzed genome-wide mRNA expression profiles under hydrostatic pressures. In this experiment, we selected a hydrostatic pressure of 30 MPa at 25 degrees C because yeast cells are able to grow with this condition. Keywords: stress response
2007-11-01 | GSE9403 | GEO
Project description:Hydrostatic pressure is one of the physical factors affecting cellular physiology. Hydrostatic pressure of a few hundred MPa decreases the viability of yeast cells, and pressure of a few tens MPa decreases the growth rate. To understand the effect of hydrostatic pressure, we employed yeast, Saccharomyces cerevisiae, DNA microarrays and analyzed genome-wide mRNA expression profiles under hydrostatic pressures. In this experiment, we selected a hydrostatic pressure of 40 MPa at 25 degrees C because the condition is not lethal for yeast cells but the growth was suppressed. Keywords: stress response
2007-11-01 | GSE9406 | GEO
Project description:Effect of different hydrostatic pressures on mouse metatarsal and hip cartilage tissues
| PRJNA980133 | ENA
Project description:Pyrococcus yayanosii CH1 is the first and only obligate piezophilic hyperthermophilic microorganism discovered so far, that extends the physical and chemical limits of life on Earth and strengthens the idea of the existence of a hyperthermophilic biosphere in the depth of our planet. It was isolated from the Ashadze hydrothermal vent at 4,100 m depth. Multi-omics analyses where performed in order to study the mechanisms implemented by the cell to face high hydrostatic pressure variations. In silico analyses showed that P. yayanosii genome is highly adapted to its harsh environment with precisely a loss of aromatic amino acid biosynthesis and the high constitutive expression of the energy metabolism compared to others non obligate piezophilic Pyrococus. Differential proteomics and transcriptomics analyses identified key hydrostatic pressure responsive genes involved in translation, chemotaxis, energy metabolism (hydrogenases and formate metabolism) and CRISPR-cas. Cells were grown at different hydrostatic pressures (20, 52 and 80 Mpa for P. yayanosii and 0.1 and 45 Mpa for P. furiosus) until they reached the middle of the exponential phase. Each culture was done 3 times independantly.
2015-09-09 | E-GEOD-72783 | biostudies-arrayexpress
Project description:Investigating protein structure, conformation, and activity under hydrostatic pressure is a critical challenge, particularly since a sizable portion of life is marine or deep subsurface. There is a perception that most proteins experience limited changes under pressures environmentally relevant on Earth; this conclusion is biased as studies focus on pressure sensitivity within a select few individual molecules, which tend to be “well-behaved”, stable proteins. More high-throughput biophysical techniques must be employed to identify promising model systems with measurable pressure effects to expand the investigation for piezo-tolerance and -sensitivity origins. Limited proteolysis (LiP) is a technique that uses differential non-specific protease susceptibility to gauge protein structure and conformational changes. When combined with mass spectrometry (MS), this method permits the profiling of subtle structural alterations across entire proteomes. The impressive throughput of LiP-MS offers a unique opportunity to survey hydrostatic pressure effects en masse. Here, we introduce the first high-pressure structural proteomics study with novel, purpose-built equipment allowing LiP-MS to be completed under ocean-bottom pressures (100 MPa). Results in a piezo-sensitive model species, Thermus thermophilus¸ show whole-proteome structural changes are rapid and more extensive than anticipated, demonstrating the capacity of high-pressure LiP-MS (HiP-LiP-MS) to profile pressure-sensitive proteins and provide a rich source of new targets for future studies.
2024-09-12 | PXD047671 | Pride
Project description:Schizophyllum commune strain:20R-7-F01 Genome sequencing and assembly
| PRJNA544166 | ENA
Project description:Series containes 4 independent experiments and high and low power scanns for each independent experiment. Genome-wide mRNA expression profiles of Saccharomyces cerevisiae growing under hydrostatic pressure were characterized. We selected a hydrostatic pressure of 30 MPa at 25°C because yeast cells were able to grow under these conditions, while cell size and complexity were increased after decompression. Functional characterization of pressure-induced genes suggests that genes involved in protein metabolism and membrane metabolism were induced. The response to 30 MPa was significantly different from that observed under lethal conditions because protein degradation was not activated under 30 MPa pressure. Strongly induced genes included those that contribute to membrane metabolism and which are also induced by detergents, oils, and membrane stabilizers. Keywords: repeat sample
2005-04-14 | GSE2526 | GEO
Project description:Series containes 4 independent experiments and high and low power scanns for each independent experiment. Genome-wide mRNA expression profiles of Saccharomyces cerevisiae growing under hydrostatic pressure were characterized. We selected a hydrostatic pressure of 30 MPa at 25°C because yeast cells were able to grow under these conditions, while cell size and complexity were increased after decompression. Functional characterization of pressure-induced genes suggests that genes involved in protein metabolism and membrane metabolism were induced. The response to 30 MPa was significantly different from that observed under lethal conditions because protein degradation was not activated under 30 MPa pressure. Strongly induced genes included those that contribute to membrane metabolism and which are also induced by detergents, oils, and membrane stabilizers.
2010-06-10 | E-GEOD-2526 | biostudies-arrayexpress
Project description:RNAseq was performed on zebrafish larvae infected with bacteria under different osmotic pressures. The trascriptome profile generated here reveals the differential immune gene expression pattern.
2018-05-15 | GSE111528 | GEO