Brucella melitensis bv. 1 str. 16M
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA110199 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Many pathogenic bacteria use a regulatory process termed Quorum Sensing (QS) to produce and detect small diffusible molecules to synchronize gene expression within a population. In Gram-negative bacteria, the detection and response to these molecules depend on transcriptional regulators belonging to the LuxR family. Such a system have been discovered in the intracellular pathogen Brucella melitensis, a Gram-negative bacteria responsible for brucellosis, a word-wide zoonosis remaining a serious public health concern in endemic countries. Two LuxR-type regulators, VjbR and BabR, have been identified in the genome of this pathogen. The vjbR mutant is highly attenuated in all tested models suggesting a crucial role of QS in the virulence of Brucella. This attenuation is at least due to the involvement of VjbR in the activation of the virB operon coding for a type four secretion system essential for Brucella to reach its intracellular replication compartment. At present, no function has been attributed to BabR. To assess the role of both Brucella QS-regulators, we performed in tandem comparative transcriptomic and proteomic analyses of vjbR and babR mutants. These experiments revealed that 10% of Brucella genome is regulated through those regulators, revealing that QS is a global regulatory system in this intracellular pathogen. The overlapping between BabR and VjbR targets suggest an unexpected cross-talk between these two regulators. Moreover, our results demonstrate that VjbR and BabR regulate many gene and/or proteins involved in stress response, metabolism and virulence. These targets are potentially involved in the adaptation of Brucella to the oxidative, pH and nutritional stresses encountered within the host. These findings highlight the involvement of QS in the virulence of Brucella and led us to suggest that this regulatory system could be implied in the spatial and sequential adaptation of Brucella to the host environment. Keywords: Quorum Sensing, Comparative gene expression, Brucella melitensis
2010-04-12 | GSE8844 | GEO
Project description:Causes brucellosis and undulant fever
| PRJNA180 | ENA
Project description:Expt 1: The contribution of vjbR and N-dodecanoyl homoserine lactone on gene regulation in Brucella melitensis
| PRJNA113989 | ENA
Project description:Brucella melitensis bv. 1 str. 16M Raw sequence reads
| PRJNA917044 | ENA
Project description:Brucella melitensis bv. 1 str. 16M Genome sequencing
| PRJNA243895 | ENA
Project description:Temporal Gene Expression Analysis of the intracellular pathogen Brucella melitensis following bovine Peyer's patch infection.
| PRJNA350161 | ENA
Project description:Differential Expression of Iron Acquisition Genes by Brucella melitensis and Brucella canis During Macrophage Infection (part 1)
| PRJNA156335 | ENA
Project description:Comparative transcriptome analysis of 16M and its vjbR mutant under a condition simulating environments of macrophage
| PRJNA131943 | ENA
Project description:Virulence effects and signaling partners modulated by Brucella melitensis light-sensing histidine kinase
| PRJNA182332 | ENA
Project description:PrlR regulon in Brucella melitensis 16M
| PRJNA151287 | ENA