Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effect of depletion of C10orf71 on gene expression in hearts from embryonic mice at E18.5 and adult mice

ABSTRACT: Effect of depletion of C10orf71 on gene expression in hearts from embryonic mice at E18.5 and adult mice

PROVIDER: PRJNA1103880 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR28785562_1.fastq.gz	Fastqsanger.gz
	SRR28785562_2.fastq.gz	Fastqsanger.gz
	SRR28785563_1.fastq.gz	Fastqsanger.gz
	SRR28785563_2.fastq.gz	Fastqsanger.gz
	SRR28785564_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 32

Similar Datasets

Effect of depletion of C10orf71 on gene expression in hearts from embryonic mice at E18.5 and adult mice

Project description:To explore the specific functional and cellular processes that affected by the mC10orf71 deletion, we compared total RNA expression in hearts from E18.5. Gene Ontology (GO) enrichment analysis showed that down-regulated genes were not significantly enriched in any biological process. The 327 genes up-regulated in KO mice were enriched for energy generation, electron transport chain and ATP synthesis, and many of them are encoded by mitochondrial DNA. These changes were likely to be a compensatory response to the impaired morphogenesis and dysfunction of the KO hearts. Then, we performed RNA-seq on hearts of adult WT and KO mice. GO analysis of down-regulated genes identified two categories of biological processes: mRNA processing/splicing and muscle cell differentiation/contraction.

2024-06-06 | GSE264733 | GEO

Transcriptome of E18.5 CHD4-M195I mouse hearts

Project description:Purpose: The goal of this study is to compare the cardiac transcriptome profiling (RNA-seq) of WT and CHD4-M195I hearts at E18.5 to conclude genes affected by this CHD4 mutation. Methods: mRNA profiles of E18.5 WT and CHD4-M195I mouse hearts were generated by deep sequencing, n=4 for each genotype, using Illumina HiSeq2500. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. Results: RNA-sequencing (RNA-seq) analyses on E18.5 WT and CHD4-M195I hearts and identified 323 genes that were differentially expressed [adjusted P value <0.05, |log2(Fold Change)| > 0.5], 113 upregulated and 210 downregulated in CHD4-M195I hearts.

2023-05-08 | GSE229050 | GEO

Transcriptome of E18.5 CHD4-M195I mouse hearts

Project description:Transcriptome of E18.5 CHD4-M195I mouse hearts

| PRJNA952639 | ENA

E18.5 DRG: Identification of a sacral, visceral sensory transcriptome in embryonic and adult mice

Project description:E18.5 DRG: Identification of a sacral, visceral sensory transcriptome in embryonic and adult mice

| PRJNA544211 | ENA

snRNA-seq of whole hearts from adult BL6 mice

Project description:snRNA-seq of whole hearts from adult BL6 mice

| PRJEB37119 | ENA

RNA-sequence analysis of WT mice hearts and cardiomyocyte-specific deletion of STAT3 mice hearts

Project description:The RNA-sequence analysis of cardiomyocyte-specific deletion of STAT3 mice hearts showed that comparing with WT mice hearts, the STAT3cKO mice hearts showed reduced cardiac function by affecting some key pathways.

2023-10-12 | GSE160248 | GEO

snRNA-seq of whole hearts from adult BL6 mice

Project description:We used snRNA-seq to investigate an entire adult mammalian heart of BL6 mice. Whole hearts were harvested from 4 male mice (12 weeks) after cervical dislocation. The hearts were pooled and nuclei isolated using the Nuclei PURE Prep isolation kit (Sigma-Aldrich, Darmstadt, Germany) according to the manufacturer’s protocol. Sequencing was conducted by Genewiz (Leipzig, Germany) on the 10xGenomics system. Single nuclei were captured in droplet emulsions and snRNA-seq libraries were constructed as per the 10x Genomics protocol using GemCode Single-Cell 3′ Gel Bead and Library V3 Kit. RNA was controlled for sufficient quality on an Agilent 2100 Bioanalyzer system and quantified using a Qubit Fluorometer.

2020-03-16 | E-MTAB-8848 | biostudies-arrayexpress

The effect of senescent cells depletion in obese hearts

Project description:The effect of senescent cells depletion in obese hearts

| PRJNA1101952 | ENA

E18.5 DRG: Identification of a sacral, visceral sensory transcriptome in embryonic and adult mice

Project description:Visceral sensory neurons encode distinct sensations from healthy organs and initiate pain states that are resistant to common analgesics. Transcriptome analysis is transforming our understanding of sensory neuron subtypes but has generally focused on somatic sensory neurons or the total population of neurons in which visceral neurons form the minority. Our aim was to define transcripts specifically expressed by sacral visceral sensory neurons, as a step towards understanding the unique biology of these neurons and potentially lead to identification of new analgesic targets for pelvic visceral pain. Our strategy was to identify genes differentially expressed between sacral dorsal root ganglia (DRG) that include somatic neurons and sacral visceral neurons, and adjacent lumbar DRG that comprise exclusively somatic sensory neurons. This was performed in male and female mice (adult and E18.5). By developing a method to restrict analyses to nociceptive Trpv1 neurons, a larger group of genes were detected as differentially expressed between spinal level. We identified many novel genes not previously been associated with pelvic visceral sensation or nociception. Limited sex differences were detected across the transcriptome of sensory ganglia, but more were revealed in sacral levels and especially in Trpv1 nociceptive neurons. These data will facilitate development of new tools to modify mature and developing sensory neurons and nociceptive pathways.

2020-03-02 | GSE131622 | GEO

In-vivo inhibition of Fgfr2b signalling in FACS-isolated AECs from embryonic mouse lungs from E16.5 to E18.5

Project description:Rosa26rtTA;tetOsFgfr2b (C75Bl6) induced from E16.5 until E18.5, lungs were harvest at E18.5, AECI and AECII were sorted using FACS. Mice were kept on water and food ad libitum.

2021-06-01 | GSE115878 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data