Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Spiroplasma endosymbiont of Polydrusus pterygomalis

ABSTRACT: Spiroplasma endosymbiont of Polydrusus pterygomalis overview

PROVIDER: PRJNA1103928 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

In Vitro Culture of the Insect Endosymbiont Spiroplasma poulsonii Highlights Bacterial Genes Involved in Host-Symbiont Interaction

Project description:Endosymbiotic bacteria associated with eukaryotic hosts are omnipresent in nature, particularly in insects. Studying the bacterial side of host-symbiont interactions is, however, often limited by the unculturability and genetic intractability of the symbionts. Spiroplasma poulsonii is a maternally transmitted bacterial endosymbiont that is naturally associated with several Drosophila species. S. poulsonii strongly affects its host’s physiology, for example by causing male killing or by protecting it against various parasites. Despite intense work on this model since the 1950s, attempts to cultivate endosymbiotic Spiroplasma in vitro have failed so far. Here, we developed a method to sustain the in vitro culture of S. poulsonii by optimizing a commercially accessible medium. We also provide a complete genome assembly, including the first sequence of a natural plasmid of an endosymbiotic Spiroplasma species. Last, by comparing the transcriptome of the in vitro culture to the transcriptome of bacteria extracted from the host, we identified genes putatively involved in host-symbiont interactions. This work provides new opportunities to study the physiology of endosymbiotic Spiroplasma and paves the way to dissect insect-endosymbiont interactions with two genetically tractable partners.

2018-03-24 | GSE112290 | GEO

icPolPter2.Spiroplasma_sp_2

Project description:Spiroplasma endosymbiont of Polydrusus pterygomalis genome assembly, icPolPter2.Spiroplasma_sp_2

| PRJEB75086 | ENA

icPolPter2.Spiroplasma_sp_1

Project description:Spiroplasma endosymbiont of Polydrusus pterygomalis genome assembly, icPolPter2.Spiroplasma_sp_1

| PRJEB75135 | ENA

Polydrusus pterygomalis

Project description:Polydrusus pterygomalis overview

| PRJNA1084997 | ENA

icPolPter

Project description:Polydrusus pterygomalis