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Characterization of IL-6R-expressing monocytes in the lung of patients with chronic obstructive pulmonary disease [THP1]

ABSTRACT: Characterization of IL-6R-expressing monocytes in the lung of patients with chronic obstructive pulmonary disease [THP1]

PROVIDER: PRJNA1104555 | ENA |

REPOSITORIES: ENA

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Characterization of IL-6R-expressing monocytes in the lung of patients with chronic obstructive pulmonary disease [THP1]

Project description:Background: Monocytes play a crucial role in innate immune responses for host defense, yet their involvement in chronic obstructive pulmonary disease (COPD) remains poorly understood. We previously identified a subset of monocytes in COPD lung tissues characterized by high interleukin-6 receptor (IL-6R) expression. This study aimed to characterize the phenotypes of IL-6Rhi monocytes in the lung of COPD patients. Methods: Using flow cytometry, we assessed the abundance of pulmonary CD14+IL-6Rhi cells in never smokers (CNS), control ex-smokers (CES) and COPD patients. IL-6 expression in CD14+ monocytes from peripheral blood of patients with COPD was also examined. CD45+CD206–CD14+IL-6Rhi and CD45+CD206–CD14+IL-6R– cells were isolated from COPD lung tissues for transcriptome analysis. A monocyte line THP1 cell with constitutive IL-6R expression was stimulated with recombinant IL-6, followed by RNA sequencing to evaluate IL-6 responsiveness of IL-6R+ monocytes. Results: The number of pulmonary CD14+IL-6Rhi monocytes was elevated in COPD patients compared to CNS, while CD14+ monocytes in the peripheral blood of COPD patients did not express IL-6R. Upregulated mRNA expression in CD14+IL-6Rhi monocytes was associated with chemotaxis, monocyte differentiation, fatty acid metabolism and integrin-mediated signaling pathway. Stimulation of THP1 cells with recombinant IL-6 induced gene expression changes linked to chemotaxis and organism development. Conclusion: In patients with COPD, CD14+IL-6Rhi monocytes increase in lung tissues compared to CNS and exhibit a different transcriptome profile from CD14+IL-6R– monocytes.

2024-08-21 | GSE265855 | GEO

Characterization of IL-6R-expressing monocytes in the lung of patients with chronic obstructive pulmonary disease [IL-6 mono]

2024-08-21 | GSE265853 | GEO

Characterization of IL-6R-expressing monocytes in the lung of patients with chronic obstructive pulmonary disease [IL-6 mono]

Project description:Characterization of IL-6R-expressing monocytes in the lung of patients with chronic obstructive pulmonary disease [IL-6 mono]

| PRJNA1104544 | ENA

Quantitative proteomic analysis of human monocytes (THP1)

Project description:To observe the changes of protein expression in human monocytes (THP1) infected with adenovirus.

2023-02-06 | PXD039874 |

Genes regulated by CREB1 in LPS-primed THP1 monocytes

Project description:To screen the genes and pathways regulated by CREB1 in the THP1 monocytes induced by LPS, THP1 monocytes were transfected by CREB1 shRNA and then treated by LPS for 3 hours.

2023-04-14 | GSE229580 | GEO

Decoding the gene promoters binding with BRD3 in LPS-primed THP1 monocytes

Project description:To screen the genes regulated by BRD3 in the THP1 monocytes induced by LPS, THP1 monocytes were treated by LPS for 1 hour in the presence or absence of OTX015

2023-12-31 | GSE234162 | GEO

RNA-seq of CYR61-treated THP1 human monocytes

Project description:We assess the effects of CYR61 treatment on the transcriptome of human THP1 monocytes.

2023-10-18 | GSE233125 | GEO

Chip-chip from THP1 cells with Pol II, Pol II Ser5-P, H3K9Ac and from primary monocytes with H3K4Me3

Project description:THP1 cells were ChIPed with indicated antibodies. Comparison of selected chromatin marks in THP1 cells and monocytes

2013-02-15 | E-GEOD-43870 | biostudies-arrayexpress

Virgibacillus sp. 6R strain:6R

Project description:Virgibacillus sp. 6R strain:6R Genome sequencing and assembly

| PRJNA349088 | ENA

RNA-seq of CYR61-treated THP1 human monocytes

Project description:RNA-seq of CYR61-treated THP1 human monocytes