Project description:Bryophytes comprise mosses, liverworts and hornworts. The chromatin landscapes of mosses and liverworts are different, leaving open the question regarding the identity of the chromatin landscape of all bryophytes. To address this question we profiled five chromatin marks using a model hornworts, Anthoceros agrestis.
Project description:Bryophytes comprise mosses, liverworts and hornworts. The chromatin landscapes of mosses and liverworts are different, leaving open the question regarding the identity of the chromatin landscape of all bryophytes. To address this question we obtained a genome wide profile of 5-methylated cytosine from a model hornwort, Anthoceros agrestis.
Project description:Heterologous expression of hornwort Rubisco from Anthoceros agrestis in Escherichia coliwith expression of Rubisco large and small subunits, chaperonins 60alpha/beta and chaperonin 20 as well as a suite of assembly factors, Raf1/Raf2/RbcX2/BSD2/RbcX1. Rubisco kinetics show differences in Rubisco assembled with and without RbcX1/2.