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Pseudomonas aeruginosa

ABSTRACT: IMP-13 producing Pseudomonas aeruginosa

PROVIDER: PRJNA1106512 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR28848656_1.fastq.gz	Fastqsanger.gz
	SRR28848657_1.fastq.gz	Fastqsanger.gz
	SRR28848658_1.fastq.gz	Fastqsanger.gz
	SRR28848659_1.fastq.gz	Fastqsanger.gz

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Project description:Pseudomonas aeruginosa PA3973 encodes a putative TetR family transcriptional regulator, with a helix-turn-helix motif involved in DNA binding. We applied phenotype analyses, as well as transcriptome profiling (RNA-seq), and genome-wide identification of binding sites using ChIP-seq to unravel the biological role of PA3973. The ChIP-seq analysis identified more than 300 PA3973 binding sites in the P. aeruginosa genome, among them 139 were located in intergenic regions. The 13 bp sequence was identified as the preferential binding site of PA3973. The PA3973 regulon encompasses genes involved in stress response, including the putative PA3973-PA3970 operon. Transcriptional profiling of P. aeruginosa PAO1161 overexpressing PA3973 showed changes in the mRNA level of 648 genes; among them, 374 were down-regulated. Concomitantly, ChIP-seq analysis identified more than 300 PA3973 binding sites in the P. aeruginosa genome, among them 139 were located in intergenic regions. The 13 bp sequence was identified as the preferential binding site of PA3973.

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