Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transfection of entomopathogenic Metarhizium species with a novel mycovirus confers hypervirulence against two lepidopteran pests

ABSTRACT: Transfection of entomopathogenic Metarhizium species with a novel mycovirus confers hypervirulence against two lepidopteran pests

PROVIDER: PRJNA1106530 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR28854278_1.fastq.gz	Fastqsanger.gz
	SRR28854278_2.fastq.gz	Fastqsanger.gz
	SRR28854279_1.fastq.gz	Fastqsanger.gz
	SRR28854279_2.fastq.gz	Fastqsanger.gz
	SRR28854280_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Transfection of entomopathogenic Metarhizium species with a novel mycovirus confers hypervirulence against two lepidopteran pests

Project description:Although most known mycoviruses are asymptomatic or reduce the virulence of their host fungi, those that confer hypervirulence to entomopathogenic fungus still need to be explored. Here, we discovered and studied a novel mycovirus in Metarhizium flavoviride, isolated from Laodelphax striatellus. Based on molecular analysis, we tentatively designated the mycovirus as Metarhizium flavoviride partitivirus 1 (MfPV1), a novel species in genus Gammapartitivirus, family Partitiviridae. MfPV1 has two double-stranded (ds) RNAs as its genome, 1,775 and 1,575 bp in size respectively, encapsidated in isometric particles. When we transfected commercial strains of M. anisopliae and M. pingshaense with MfPV1, conidiation was significantly enhanced (t-test; P-value < 0. 01), and the significantly higher mortality rates of the larvae of Plutella xylostella and Spodoptera frugiperda, two important lepidopteran pests were found in virus-transfected strains (ANOVA; P-value < 0.05). Transcriptomic analysis showed that transcript levels of pathogenesis-related genes in MfPV1-infected M. anisopliae were obviously altered, suggesting increased production of metarhizium adhesin-like protein, hydrolyzed protein and destruxin synthetase. Further studies are required to elucidate the mechanism whereby MfPV1 enhances the expression of pathogenesis-related genes and virulence of Metarhizium to lepidopteran pests. This study presents experimental evidence that the transfection of other entomopathogenic fungal species with a mycovirus can confer significant hypervirulence and provides a good example that mycoviruses could be used as synergistic agent to enhance the biocontrol activity of entomopathogenic fungi.

2024-05-03 | GSE266301 | GEO

Small RNA profiles of mycovirus-free and mycovirus-infected Aspergillus fumigatus isolates, created using ScriptMiner adapters

Project description:Small RNA libraries of 4 different mycovirus-infected and -free isogenic lines of A. fumigatus were constructed using Scriptminer adapters. sRNAomes were analysed and putative virus-derived small RNAs were identified. Analysis of small RNA profiles of 4 different mycovirus-infected A. fumigatus isolates

2017-05-02 | E-GEOD-61681 | biostudies-arrayexpress

Small RNA profiles of mycovirus-free and mycovirus-infected Aspergillus fumigatus isolates, created using ScriptMiner adapters

2017-05-02 | GSE61681 | GEO

Endogenized hypervirulence-associated DNA virus BdDV-1 is primarily restricted to enzootic lineages of the amphibian pathogen Batrachochytrium dendrobatidis

Project description:The Global Panzootic Lineage (GPL) of the pathogenic fungus Batrachochytrium dendrobatidis (Bd) has caused severe amphibian population declines, yet the drivers underlying the high frequency of GPL in regions of amphibian decline are unclear. Using publicly available Bd genome sequences, we identified multiple non-GPL Bd isolates that contain a circular Rep-encoding single stranded DNA-like virus which we named BdDV-1. We further sequenced and constructed genome assemblies with long read sequences to find that the virus is integrated into the nuclear genome in some strains. Attempts to cure virus positive isolates were unsuccessful, however, phenotypic differences between naturally virus positive and virus negative Bd isolates suggested that BdDV-1 decreases the growth of its host in vitro but increases the virulence of its host in vivo. BdDV-1 is the first described CRESS DNA mycovirus associated with hypervirulence with a distribution inversely associated with the emergence of the panzootic lineage.

2023-11-05 | GSE246809 | GEO

Small RNA profiles of three different mycovirus-infected Aspergillus fumigatus isolates, created using Illumina high definition adapters

Project description:Small RNA libraries of three different mycovirus-infected A. fumigatus isolates were constructed by using Illumina high definition adapters. sRNAomes were analysed and putative virus-derived small RNAs were identified. Analysis of small RNA profiles of three different mycovirus-infected A. fumigatus isolates

2017-05-03 | E-GEOD-61680 | biostudies-arrayexpress

Small RNA profiles of three different mycovirus-infected Aspergillus fumigatus isolates, created using Illumina high definition adapters

2017-05-03 | GSE61680 | GEO

De novo assembly in defense against pests in Pinus massoniana

Project description:We sequenced mRNA from the insect-resistant and poor insect resistance Pinus massoniana to discover metabolic pathways and genes that are involved in defense against pests.

2015-08-24 | GSE72294 | GEO

Fusarium odoratissimum mycovirus

Project description:Metagenomic assembly of Fusarium oxysporum f. sp. cubense Tropical Race 4 Mycovirus in Malaysia

| PRJNA1096116 | ENA

De novo assembly in defense against pests in Pinus massoniana

Project description:We sequenced mRNA from the insect-resistant and poor insect resistance Pinus massoniana to discover metabolic pathways and genes that are involved in defense against pests. Examination of mRNA levels in strain insect-resistant and poor insect resistance Pinus massoniana

2015-08-24 | E-GEOD-72294 | biostudies-arrayexpress

DNA microarray data from transgenic rice KMD and its parent XS11

Project description:KMD is genetically engenered to be highly resistant to lepidopteran pests through expressing a synthetic cry1Ab gene and its parent non-transgenic rice is Xiushui 11 (XS11). Many unintended effects have been discovered in KMD. We used microarrays to study the molecular basis for unintended effects of KMD rice.

2011-10-26 | GSE33203 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data