Project description:The human cytomegalovirus (HCMV) encodes the chemokine receptor US28 that exhibits constitutive activity. NIH-3T3 cells stably transfected with US28 present a pro-angiogenic and transformed phenotype both in vitro and in vivo. We used gene expression profiling to determine how US28 constitutive activity modulates expression of genes compared to mock-transfected cells. Experiment Overall Design: We isolated RNA from two different clonal stable cell lines of NIH-3T3 cells transfected either either mock or US28-WT and analysed these 4 RNA samples using Affymetrix mouse genome arrays.
Project description:The human cytomegalovirus (HCMV) encodes the chemokine receptor US28 that exhibits constitutive activity. NIH-3T3 cells stably transfected with US28 present a pro-angiogenic and transformed phenotype both in vitro and in vivo. We used gene expression profiling to determine how US28 constitutive activity modulates expression of genes compared to mock-transfected cells. Keywords: Comparison of stably transfected cell lines
Project description:Analysis of gene expression in NIH 3T3 cells stably knockdown for Selt (GenBank accession number NM_001040396) using vector based siRNA technique in comparison to gene expression of vector transfected NIH 3T3 cells.
Project description:ATCE1 is a transcription factor that belongs to the CREB3 subtype of the ATF/CREB transcription factor family. In order to get an indication of the potential promoters ATCE1 targets, a nuclear form was expressed in NIH 3T3 fibroblasts via transfection and upregulated genes were sought after. Experiment Overall Design: NIH 3T3 cells were transfected with either a pCMV-HA-ATCE1-C-del plasmid, driving the expression the nuclear form of ATCE1, or an empty pCMV-HA vector. Experiment Overall Design: The expression level of transcripts isolated from ATCE1 expressing cells was compared to transcripts obtained from cells transfected with an empty vector that was used as a control.
Project description:U87 cell lines were stable transfected with C19ORF63 (Human hematopoietic peptide secreted-1 - HSS1). HSS1 is a truly novel protein defining a new class of secreted factors. U87 cell line overexpressing HSS1 greatly reduced their proliferation rate compared to mock-transfected cells. Microarray analysis was used to detail gene expression underlying the anti-proliferative and anti-tumorigenic effect of HSS1 in U87 cells. Exponentially growing U87 cells at growth curve day 5 were harvested for total RNA extraction and hybridization on Affimetrix microarrays. Three groups of samples were evaluated in triplicates: U87 wild-type, U87 -pcDNA3.1 mock-transfected, U87-pcDNA-HSS1. Cells were stable transfected with pcDNA3.1 empty vector or hHSS1. hHSS1-expressing cells and control cells were at confluence 40-80% when harvested. Trypan blue analysis of the number of viable cells showed a significant anti-proliferative effect in U87 cells expressing hHSS1 as compared to the control cells.
