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Breast organoid suspension cultures maintain long-term estrogen receptor expression and responsiveness

ABSTRACT: Breast organoid suspension cultures maintain long-term estrogen receptor expression and responsiveness

PROVIDER: PRJNA1108743 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28928300_1.fastq.gz	Fastqsanger.gz
	SRR28928300_2.fastq.gz	Fastqsanger.gz
	SRR28928301_1.fastq.gz	Fastqsanger.gz
	SRR28928301_2.fastq.gz	Fastqsanger.gz
	SRR28928302_1.fastq.gz	Fastqsanger.gz

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Breast organoid suspension cultures maintain long-term estrogen receptor expression and responsiveness

Project description:Organoid cultures offer a powerful technology to investigate many different aspects of development, physiology, and pathology of diverse tissues. Unlike standard tissue culture of primary breast epithelial cells, breast organoids preserve the epithelial lineages and architecture of the normal tissue. However, existing organoid culture methods are tedious, difficult to scale, and do not robustly retain estrogen receptor (ER) expression and responsiveness in long-term culture. Here, we describe a modified culture method to generate and maintain organoids as suspension cultures. This method improves organoid growth and uniformity over the traditional Matrigel dome embedding method while still maintaining the fidelity of the three major epithelial lineages. Using this adopted method, we are able to isolate and culture hormone sensing (HS) cells alone that retain ER responsiveness upon estrogen stimulation in long-term culture. This culture system presents a valuable opportunity to study the events involved in initiation and evolution of ER-positive breast cancer.

2024-12-27 | GSE266935 | GEO

Single-cell transcriptome atlas of human retinal organoid development

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2023-03-12 | E-MTAB-12715 | biostudies-arrayexpress

Single-cell chromatin accessibility atlas of human retinal organoid development

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2023-03-12 | E-MTAB-12714 | biostudies-arrayexpress

Stirred suspension bioreactors maintain naïve pluripotency of human pluripotent stem cells (hPSCs)

Project description:Stirred suspension bioreactors maintain naïve pluripotency of human pluripotent stem cells (hPSCs)

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Kidney micro-organoids generated in suspension culture

Project description:Kidney organoids have potential uses in disease modelling, drug screening and regenerative medicine. However, novel cost-effective techniques are needed to enable scale-up production of kidney cell types in vitro. We describe here a modified suspension culture method for the generation of kidney micro-organoids from human pluripotent stem cells. Each micro-organoid contains 6-10 nephrons surrounded by endothelial and stromal populations. Single cell transcriptional profiling confirmed the presence and transcriptional equivalence of all anticipated renal cell types consistent with a previous organoid culture method. Ligand-receptor mapping identified TGFβ signalling between stromal and epithelial cell types as likely to result in fibrotic changes observed with long-term culture. The addition of an ALK4 inhibitor suppressed stromal expansion, maintaining epithelial morphology and improving maturation. This suspension culture micro-organoid methodology resulted in a 3-4-fold increase in final cell yield compared to static culture, thereby representing an economical approach to the production of kidney cells for various biological applications.

2019-03-01 | GSE117211 | GEO

Fine tuning of epithelial taste bud organoid to promote functional recapitulation of taste reactivity

Project description:Taste stem/progenitor cells from the mouse posterior tongue have been recently used to generate taste bud organoids. However, the inaccessible location of the taste receptor cells is observed in conventional organoids. Here, we established a suspension culture method for fine tuning of taste bud organoid by apicobasal polarity alteration to form the accessible localization of taste receptor cells in organoid. Compared to conventional Matrigel-embedded organoids, suspension-cultured organoids showed comparable differentiation and renewal rates to those of taste buds in vivo and exhibited functional taste receptor cells and cycling progenitor cells. Accessible taste receptor cells on the outer region of taste bud organoids enabled the direct application of calcium imaging for evaluating the taste response. Moreover, suspension-cultured organoids could be genetically altered using gene editing methods. Suspension-cultured taste bud organoid harmoniously integrated with the recipient lingual epithelium; maintained the taste receptor cells and gustatory innervation capacity. Thus, we propose that suspension-cultured organoids may provide efficient model for taste research including taste bud development, regeneration and transplantation

2021-12-25 | GSE191169 | GEO

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Project description:Endometrial gland cultures were established from human non-pregnant endometrium and decidua. We analysed the global gene expression profile of the human endometrial gland organoid cultures to assess the similarity of their molecular signature to the tissue of origin. Organoid cultures established from decidua were also included in the analysis to assess their similarity to endometrial dervied cultures.

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Gene expression of B cell differentiation in Organoid Cultures

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2024-08-01 | GSE268955 | GEO

Stirred suspension bioreactors maintain naïve pluripotency of human pluripotent stem cells (hPSCs)

Project description:Although cell therapies require large numbers of quality-controlled hPSCs, existing technologies are limited in their ability to efficiently grow and scale stem cells. We report here that cell-state conversion from primed-to-naïve pluripotency enhances the biomanufacturing of hPSCs. Naïve hPSCs exhibit superior growth kinetics and aggregate formation characteristics in stirred suspension bioreactors compared to their primed counterparts. Moreover, we demonstrate the role of the bioreactor mechanical environment in the maintenance of naïve pluripotency, through transcriptomic enrichment of mechano-sensing signaling for cells in the bioreactor along with a decrease in expression of lineage-specific and primed pluripotency hallmarks. Bioreactor-cultured, naïve hPSCs maintain epigenetic hallmarks of naïve pluripotency, exhibit robust production of naïve pluripotency metabolites, and display reduced expression of primed pluripotency cell surface markers. We also show that these cells retain the ability to undergo targeted differentiation into beating cardiomyocytes, hepatocytes and neural rosettes. They additionally display faster kinetics of teratoma formation compared to their primed counterparts. Naïve bioreactor hPSCs also retain structurally stable chromosomes. Our research corroborates that converting hPSCs to the naïve state enhances hPSC manufacturing and indicates a potentially important role for the bioreactor’s mechanical environment in maintaining naïve pluripotency.

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