Project description:Here, we report the genomic-scale characterization of locally advanced colon cancer transcriptome. Paraffin embedded samples was used to asses differences between normal colon, primary colon tumor an lymph node metastasis.
Project description:In order to have a comprehensive comparison of locally restricted mpeg1+ cells (MLCs) and cLCs, we isolated locally restricted mpeg1+ cells and cLCs from heat-shock labeled zebrafish epidermis and performed whole-transcriptome RNA-sequencing. As the controls, keratinocytes, T cells and neutrophils isolated from the epidermis were also included in this experiment. Both T-SNE analysis and feature gene comparison indicate that locally restricted mpeg1+ cells indeed share the highest similarity with cLCs in transcriptome profile.
