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The primitive endoderm supports lineage plasticity to enable regulative development [scRNA-Seq]

ABSTRACT: The primitive endoderm supports lineage plasticity to enable regulative development [scRNA-Seq]

PROVIDER: PRJNA1110211 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28992003.fastq.gz	Fastqsanger.gz
	SRR28992004.fastq.gz	Fastqsanger.gz
	SRR28992005.fastq.gz	Fastqsanger.gz
	SRR28992006.fastq.gz	Fastqsanger.gz
	SRR28992007.fastq.gz	Fastqsanger.gz

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Project description:Mammalian blastocyst formation involves the specification of trophectoderm followed by differentiation of the inner cell mass into embryonic epiblast and extra-embryonic primitive endoderm (PrE). During this time, the embryo maintains a window of plasticity and can redirect its cellular fate when challenged experimentally. In this context, we found that the PrE alone was sufficient to regenerate a complete blastocyst and continue postimplantation development. We identify an in vitro population similar to the early PrE in vivo that exhibits the same embryonic and extra-embryonic potency and can form complete stem cell-based embryo models termed blastoids. Commitment in the PrE is suppressed by JAK/STAT signalling, collaborating with OCT4 and the sustained expression of a subset of pluripotency-related transcription factors that safeguard an enhancer landscape permissive for multi-lineage differentiation. Our observations support the notion that transcription factor persistence underlies plasticity in regulative development and highlights the importance of the PrE in perturbed development.

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