Project description:This study compares the transcriptomes of wild (Figgjo) and domesticated (Mowi) Atlantic salmon embryos, employing a custom 44k oligonucleotide microarray to identify gene pathways perturbed between wild and domesticated strains and with the aid of heir reciprocal hybrids, to examine the heritability of differentially expressed genes.
Project description:Domesticated animals share a unique set of morphological and behavioural traits, jointly referred to as the domesticated phenotype. These include modified growth, reproduction, metabolism, pubertal development and stress response. Striking similarities amongst a range of unrelated domesticated species suggest that similar regulatory mechanisms may underlie the domesticated phenotype. Several previous studies have focused on the brain to find mechanisms underlying domestication effects on the stress response, whereas the potential role of the pituitary gland as a target of domestication is highly overlooked. Here, we study gene expression in the pituitary gland of the domesticated White Leghorn chicken and its ancestor, the Red Junglefowl. We exposed chicken of both breeds to 15 minutes of restraint stress. We then culled the animals and dissected out the pituitary gland and snap froze them. RNA was later extracted from the pituitaries and gene expression was measured using Agilent microarray.
Project description:A 44K salmonid oligoarray was used to examine the influence of genotype and developmental stage on mRNA expression in wild-type (age matched and size matched to domesticated), domesticated and wild-domesticated hybrid populations. Significant differences were found in mRNA expression between genotypes. The largest proportion of differentially expressed mRNAs were found in comparisons of domesticated and wild-type groups. Significant differences were also noted in pairings of wild-domesticated hybrid progeny to both parental strains. Differences in gene expression were found in comparison of both wild-type age and size matched to each other and in combination with genotype analysis, showing a strong effect of fish developmental stage on differential gene expression. Domestication was found to modify biological pathways associated with transport, metabolism, cell tissue structure and development, stress and immune response and protein synthesis. Genotype analysis of hybrid progeny inheritance patterns showed similar levels of recessive, dominant and additive effects. Microarray analysis was performed on liver RNA from 6 individual fish per group of age-matched wild, size-matched wild, domesticated and wild-domesticated hybrids. Samples were hybridized against a common wild-type reference pool (n=6 wild size-matched fish). One individual from each of the groups was hybridized to one array on each of the individual slides (n=6) in the order of domestic, hybrid, age-matched wild and size-matched wild. In total, 6 4x44K oligoarray slides were used to complete the study.
Project description:This study compares the transcriptomes of wild and domesticated Atlantic salmon strains and their transcriptional response to acute stress. Microarray interrogations consisted of 48 hybridizations; 4 crosses (pure wild; Figgjo x Figgjo, pure domesticated; Mowi x Mowi and their reciprocal hybrids; Figgjo x Mowi, Mowi x Figgjo) x 2 conditions (stress and control) x 6 biological replicates and employed a custom 44k oligonucleotide microarray design.
Project description:Gamete quality is one of the most important element of successful aquaculture. This especially applies to a newly domesticated fish species, such as pikeperch (Sander lucioperca) in which lowered and/or variable egg quality is one of the biggest obstacles toward rapid production expansion. However, mechanisms underlying pikeperch egg developmental competences remain unknown. Therefore, a quantitative proteome study was performed with high (HQ) and low (LQ) quality domesticated pikeperch eggs using a label free LC-MS/MS approach.
Project description:A 44K salmonid oligoarray was used to examine the influence of genotype and developmental stage on mRNA expression in wild-type (age matched and size matched to domesticated), domesticated and wild-domesticated hybrid populations. Significant differences were found in mRNA expression between genotypes. The largest proportion of differentially expressed mRNAs were found in comparisons of domesticated and wild-type groups. Significant differences were also noted in pairings of wild-domesticated hybrid progeny to both parental strains. Differences in gene expression were found in comparison of both wild-type age and size matched to each other and in combination with genotype analysis, showing a strong effect of fish developmental stage on differential gene expression. Domestication was found to modify biological pathways associated with transport, metabolism, cell tissue structure and development, stress and immune response and protein synthesis. Genotype analysis of hybrid progeny inheritance patterns showed similar levels of recessive, dominant and additive effects.