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ABSTRACT: Polyhydroxyalkanoates production by mixed methanotrophic culture

PROVIDER: PRJNA1111824 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29036801_1.fastq.gz	Fastqsanger.gz
	SRR29036802_1.fastq.gz	Fastqsanger.gz
	SRR29036803_1.fastq.gz	Fastqsanger.gz
	SRR29036804_1.fastq.gz	Fastqsanger.gz
	SRR29036805_1.fastq.gz	Fastqsanger.gz

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Project description:Enrichment of mixed methanotrophic cultures producing polyhydroxyalkanoates (PHAs)

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Proteomic investigation of a mixed microbial culture synthesizing polyhydroxyalkanoates from fermented dairy manure under aerobic feast-famine conditions

Project description:Polyhydroxyalkanoates (PHAs) are bio-based, biodegradable polyesters that can be produced from organic-rich waste streams using mixed microbial cultures. To maximize PHA production, mixed microbial cultures may be enriched for PHA-producing bacteria with a high storage capacity through the imposition of cyclic, aerobic feast-famine conditions in a sequencing batch reactor (SBR). Though enrichment SBRs have been extensively investigated a bulk solutions-level, little evidence at the proteome level is available to describe the observed SBR behavior to guide future SBR optimization strategies. As such, the purpose of this investigation was to characterize proteome dynamics of a mixed microbial culture in an SBR operated under aerobic feast-famine conditions using fermented dairy manure as the feedstock for PHA production. At the beginning of the SBR cycle, excess PHA precursors were provided to the mixed microbial culture (i.e., feast), after which followed a long duration devoid of exogenous substrate (i.e., famine). Two-dimensional electrophoresis was used to separate protein mixtures during a complete SBR cycle, and proteins of interest were identified.

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Project description:Multi-stage Process for Mixed Microbial Culture Production of Polyhydroxyalkanoates from Sugarcane Stillage: Assessment of External Nutrient Supplementation

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metabolic tuning of mixed purple phototrophic bacteria biofilms in photoheterotrophic conditions

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