ABSTRACT: Enhancing treatment performance of Chlorella pyrenoidosa on levofloxacin wastewater through microalgae-bacteria consortia: mechanistic insights using the transcriptome
Project description:Enhancing treatment performance of Chlorella pyrenoidosa on levofloxacin wastewater through microalgae-bacteria consortia: mechanistic insights using the transcriptome
Project description:To investigate the mechanism by which the microalgae-yeast co-culture system promotes wastewater denitrification. We concluded that microalgae and yeast exhibit a mutually beneficial relationship in the co-culture system. Microalgae nitrogen metabolism can be influenced by both miRNA and mRNA, and the presence of yeast stimulates gene expression in microalgae.
Project description:Varying levels of nitrogen deprivation can improve lipid accumulation differently in microalgae, whose underlying regulations are unknown. To investigate the related mechanisms, Auxenochlorella pyrenoidosa was exposed to 1.5, 1, 0.5 and 0 g/L sodium nitrate (NaNO3) for 5 days and RNA-seq were detected.
Project description:The understanding of molecular events occurring in Chlorella during heterotrophy to photoautotrophy transition as well as sudden light stress and glucose starvation, are still largely unknown. To well grasp its cellular metabolism, particularly the regulation of biosynthesis and degradation pathways of lipid, protein and carbohydrates, as well as the diverse trophic adaptation affecting carbon partitioning during heterotrophy to photoautotrophy transition process, we sequenced the transcriptome (RNA-seq) in six time points to discover how transcriptional changes in C. pyrenoidosa modulate metabolic flux trends leading to intracellular components dynamic reassortment.
