Project description:The mitogenome sequence of a marine gastropod, Umbonium thomasi (Crosse, 1863) (Trochida, Trochidae), was determined first in the genus Umbonium. The assembled mitogenome, consisting of 15,998?bp in length, has 13 protein-coding genes, 22 transfer RNA genes, and two ribosomal RNA genes. The order and structure of the genes are similar to those of other Trochid species. The overall base composition of mitogenome is 34.8% A, 22.1% C, 14.0% G, and 29.1% T with an A?+?T bias of 64%. The complete mitogenome of U. thomasi provides essential and important DNA molecular data for further phylogenetic and evolutionary analysis of marine gastropods.
Project description:The Thomas's horseshoe bat (Rhinolophus thomasi) is restricted to southeast Asia and few studies have been conducted on this species. Here we characterized the complete mitochondrial genome of R. thomasi using next-generation sequencing and Sanger sequencing. The whole mitogenome is 16,899 bp in length and contains 22 tRNA, 2 rRNA, 13 protein-coding genes and a non-coding control region. The tissue used in this study was taken from a sample collected 13 years ago and the genomic DNA was seriously degraded. Our study adds to a growing number of studies indicating that next-generation sequencing is powerful in generating genetic/genomic resources from museum samples.
| S-EPMC7800769 | biostudies-literature
Project description:Sequence capture for R. sinicus and R. thomasi
