Project description:modENCODE_submission_4082 This submission comes from a modENCODE project of Kevin White. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: The White Lab is aiming to map the association of all the Transcription Factors (TF) on the genome of Drosophila melanogaster. One technique that we use for this purpose is chromatin immunoprecipitation coupled with deep sequencing (ChIP-seq) utilizing an Illumina next generation sequencing platform. The data generated by ChIP-seq experiments consist basically of a plot of signal intensity across the genome. The highest signals correspond to positions in the genome occupied by the tested TF. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-seq. BIOLOGICAL SOURCE: Strain: Y cn bw sp; Developmental Stage: Adult Female; Genotype: y[1] oc[R3.2]; Gr22b[1] Gr22d[1] cn[1] CG33964[R4.2] bw[1] sp[1]; LysC[1] lab[R4.2] MstProx[1] GstD5[1] Rh6[1]; Sex: Female; EXPERIMENTAL FACTORS: Developmental Stage Adult Female; Strain Y cn bw sp; Antibody KW0-CNC (target is cap'n collar)
Project description:Purpose: The goal of this study is to identify the sex differential miRNA tanscriptome between male and female adult (4-6 wk. old) mouse hearts using small RNA-seq. Methods: miRNA profiles of male and female mouse hearts were generated by sequencing, n=4 for each sex, using Illumina HiSeq2500. The sequence reads were aligned to the mm10 reference genome using STAR via the bcbio-nextgen RNA-sequencing pipeline. Differential gene expression was determined by genwiz. Results: 11 miRNAs were differentially expressed between male and female mice [ P value <0.05, |log2(Fold Change)| > 0.5], with 9 more highly expressed in female hearts and 2 more highly expressed in male hearts.