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Longitudinal DNA methylation in serially passaged BJ Fibroblasts to investigate the latent aging of cells

ABSTRACT: Longitudinal DNA methylation in serially passaged BJ Fibroblasts to investigate the latent aging of cells

PROVIDER: PRJNA1114759 | ENA |

REPOSITORIES: ENA

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Longitudinal DNA methylation in serially passaged primary and modified human astrocytes

Project description:This DNA methylation dataset describes epigenomic changes in in vitro serially passaged primary and immortalized astrocytes, in the context of studies examining cellular aging patterns that are conserved in vivo and in vitro. Primary and fetal hTERT-immortalized astrocytes were grown under normoxic conditions and serially passaged. Longitudinal DNA samples were collected throughout passaging and DNA methylation was measured using the Infinium HumanMethylation850 BeadChip.

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Longitudinal DNA methylation in serially passaged BJ Fibroblasts to investigate the latent aging of cells

Project description:As epigenetic clocks have evolved from powerful estimators of chronological aging to predictors of mortality and disease risk, it begs the question of what role DNA methylation plays in the aging process. We hypothesize that while it has the potential to serve as an informative biomarker, DNA methylation could also be a key to understanding the biology entangled between aging, (de)differentiation, and epigenetic reprogramming. Here we use an unsupervised approach to analyze time associated DNA methylation from both in vivo and in vitro samples to measure an underlying signal that ties these phenomena together. We identify a methylation pattern shared across all three, as well as a signal that tracks aging in tissues but appears refractory to reprogramming, suggesting that aging and reprogramming may not be fully mirrored processes.

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2018-10-22 | PXD007732 | Pride

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