Project description:We show that dHNF4 null mutant larvae are sensitive to starvation. Starved mutant larvae consume glycogen normally, but retain lipids in their midgut and fat body, and have increased levels of long chain fatty acids, suggesting that they are unable to efficiently mobilize stored fat for energy. HNF4 dependent transcripts were identified under fed and starved conditions. Microarray studies indicate reduced expression of genes that control lipid catabolism and ï¢-oxidation. Late second instar control and HNF4 mutant larvae were collected and fed or starved for 24 hours, and then RNA was isolated for analysis. Taken together, our results support a feed-forward model for dHNF4, in which fatty acids released from triglycerides activate the receptor, inducing enzymes that drive fatty acid oxidation for energy production. Experiment Overall Design: Staged late second instar control larvae that were transheterozygous for precise excisions of the EP2449 and KG08976 P-elements, and dHNF4D33/dHNF4D17 mutant larvae, were either fed or starved for 24 hrs in a 55mm petri dish containing Whatman paper soaked with PBS. RNA was extracted from the apparently healthy animals that molted to the third instar using Trizol (Gibco) and purified on RNAeasy columns (Qiagen). All samples were prepared in triplicate to facilitate subsequent statistical analysis. Probe labeling, hybridization to Affymetrix GeneChipÂ® Drosophila Genome 2.0 Arrays.

2009-01-31 | E-GEOD-14531 | biostudies-arrayexpress

Drosophila melanogaster

Project description:Sex-biased gene expression in Drosophila melanogaster larvae

| PRJNA145347 | ENA

Drosophila melanogaster

Project description:Ionizing Radiation-induced expression response in Drosophila Larvae

| PRJNA159779 | ENA

Drosophila melanogaster

Project description:Transcriptional and ribosome profiling of Drosophila 3rd instar larvae muscle

| PRJNA390134 | ENA

Drosophila melanogaster

Project description:Expression data from Drosophila third instar larvae eye imaginal discs