Project description:To identify novel genes modulating Candida albicans biofilm formation, a screen of 2451 overexpression strains allowed us to identify 16 genes whose overexpression significantly reduced biofilm formation. Genome-wide expression and binding analyses were conducted upon overexpression of ZCF15 and ZCF26 and wild type planktonic and biofilm cells were performed. A ChIP assays was performed. Briefly, untagged strain (CEC4665) and two replicates each of ZCF15 (CEC5929 and CEC5930) and ZCF26 (CEC5931 and CEC5932) strain were grown in biofilm condition for 18 h and cells were cross-linked with 1% final concentration of formaldehyde for 25 min at 30°C.The DNA was immunoprecipitated with anti-protein A antibodies (Sigma Aldrich Cat. No. P3775). The immunoprecipitated (IP) DNA were used to determine the binding of Zcf15 and Zcf26 across the genome by ChIP-sequencing
