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ABSTRACT:
PROVIDER: PRJNA1117076 | ENA |
REPOSITORIES: ENA
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Project description:In this study, an artificial feeding study containing (E)-β-farnesene (0.8g/kg) with C. suppressalis larvae was conducted to investigate its physical performance, and results revealed that the average 2nd instar duration in C. suppressalis significantly increased from 4.78 days to 6.31 days after Eβf treatment (P<0.001), and in 3rd instar C. larvae also significantly increased from 5.70 days to 8.00 days (P<0.001). There were no significant differences in 4th and 5th C. suppressalis larval between Eβf treatment and control(P>0.05). The mortality rates of 2nd C. suppressalis larvae were increased by 21.00-fold, compared with control(P<0.001), 3rd C. suppressalis larval increased by 6.39-fold(P<0.001). Comparative transcriptome analysis also showed that multiple DEGs involved in insect hormone biosynthesis pathway, the results of hormone assay in 3rd instar larvae showed the balance between juvenile hormones and ecdysteroids was disrupted. Eβf treatment increased juvenile hormones titers, but not the ecdysteroids. The results of qPCR were consistent with RNA-seq. Taken together, Eβf impairs the development and survival of C. suppressalis larvae by disrupting insect hormone balance. Moreover, the altered pathways were associated with carbohydrates, xenobiotics and cofactors and vitamins in C. suppressalis larvae. Altogether, our findings identify Eβf impair the development and survival of C. suppressalis larvae. Additionally, our data also revealed a potential mechanism that Eβf impaired the development and survival of C. suppressalis larval by disrupting insect hormone balance. These findings may contribute to the integrated control of C. suppressalis.
2022-05-04 | GSE179532 | GEO
Project description:Mamestra configurata whole head transcriptome
| PRJNA596858 | ENA
Project description:Social caste determination in the honey bee is assumed to be determined by the dietary status of the young larvae and translated into physiological and epigenetic changes through nutrient-sensing pathways. We have employed microRNA gene-microarray, and observed that both worker jelly and royal jelly showed dynamic changes in miRNA content during the 4th to 6th day of larval development . Adding specific miRNAs to royal jelly elicited significant changes in queen larval mRNA expression and in morphological characters of the emerging adult queen bee. We propose that miRNAs in the nurse bee secretions constitute an additional element in the regulatory control of caste determination in the honey bee. We collected worker and royal jelly of the Italian honeybee (ZND No.1, Apis mellifera ligustica) at 73~90 hours (4th-day larvae), 97~114 hours (5th-day larvae), and 121~138 hours (6th-day larvae) after hatching. After total RNA was extractedM-BM- and quantified , equal amounts of total RNAs from each of the three sampling days were analyzed on the LC Science miRNA-array to observe the expression variation of miRNAs between worker jelly and royal jelly along with the development time points (4th-day, 5th-day and 6th-day).
2013-12-31 | E-GEOD-50457 | biostudies-arrayexpress
Project description:We wished to investigate if adaptation to host-plant diet is the basis of differentiation for two strains of Spodoptera frugiperda (Lepidoptera:Noctuidae). We performed reciprocal transplant experiments in laboratory conditions, feeding each strain (sf-C and sf-R) with artificial diet, corn plants or rice plants. RNA-Seq was performed on pooled 4th instar larvae from this experiment. We compared this transcriptional response with that of individual 4th instar larvae collected in corn and grass fields in Florida.
2019-02-11 | E-MTAB-6540 | biostudies-arrayexpress
Project description:Transcriptional analysis of sex-regulated genes from male and female dsxM-^VeGFP sexed larvae (1st instar, late 2nd /early 3rd instar and 4th instar), pupae and virgin non-blood-fed 3 day old adult A. gambiae mosquitoes
2011-05-01 | E-MEXP-3093 | biostudies-arrayexpress
Project description:In the silkworm, Bombyx mori, juvenile hormone (JH) and 20-hydroxyecdysone (20E) levels are high during the final larval molt (4M) but both absent during the feeding stage of 5th instar (5F), while JH level is low and 20E level is high during the prepupal stage (PP). Fat body is the important organs in insect, we want to find out differentially expressed genes which are respectively regulated by the two hormones. Total RNA from 4th molting,5th feeding and prepupa stages Bombyx fat body were used to generate target cDNA, and then hybridized to 48k Bombyx genome Array Genechips, representing about 23000 characterized genes
2010-08-04 | E-GEOD-23424 | biostudies-arrayexpress
Project description:Bombyx batryticatus, the dried larva of Bombyx mori L. (4th–5th instars) infected with Beauveria bassiana Vuill, is an important animal-derived medicine effective against several diseases. The metamorphosis of silkworm can result insignificant changes in the levels of proteins and polypeptides in the 4th and 5th instar larvae. Here, we performed extensive characterization of Bombyx batryticatus peptides, including polypeptides containing cysteines, using an MS-based data mining strategy. A total of 779 peptides with various PTMs (post-translational modifications) were identified through database search and de novo sequencing. Some of these peptides might have important biological activities. Besides, the differential analysis of polypeptides between the head and body of Bombyx batryticatus was performed to provide a clinical basis for rational use of the drugs derived from it. This study illustrates the abundance and sequences of endogenous Bombyx batryticatus polypeptides, and thus, provides potential candidates for the screening of active compounds for future biological research and drug discovery studies.
2018-08-30 | PXD014281 |
Project description:Overall, the study aims at obtaining a comprehensive picture of the African malaria mosquito, Anopheles gambiae, transcriptome using high-coverage RNA-seq of sexed whole-insect samples collected at different developmental time points. This experiment focuses on male and female transcriptomes from 20 hour old embryos, 12 hour old 3rd instar larvae, 24 hour old 4th instar larvae and 10 hour old pupae sampled using a strand-specific RNA-seq approach.
2015-06-15 | E-MTAB-2880 | biostudies-arrayexpress
Project description:Using RNAseq we report differentially expressed genes after knockdown of Ataxin-2 in Drosophila S2 lysate and Drosophila 3rd instar larvae brain
2021-12-29 | GSE185473 | GEO