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Bacteroides thetaiotaomicron VPI-5482

ABSTRACT: Coordinate regulation of glycan degradation and polysaccharide capsule biosynthesis by a prominent human gut symbiont

PROVIDER: PRJNA111713 | ENA |

REPOSITORIES: ENA

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Bacteroides thetaiotaomicron VPI-5482

Project description:Regulated expression of polysaccharide utilization and capsular biosynthesis loci in biofilm and planktonic Bacteroides thetaiotaomicron during growth in chemostats

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Bacteroides thetaiotaomicron VPI-5482

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Genome-wide RNA-seq of Bacteroides thetaiotaomicron VPI-5482 with Bacteroides phage challenge

Project description:Purpose: Examining the transcriptome of Bacteroides thetaiotaomicron VPI-5482 challenged with Bacteroides phage to assess surface molecule expression changes Methods: Bacteroides thetaiotaomicron was grown in BPRM in vitro or Germ-Free mice were monocolonized with Bacteroides thetaiotaomicron and gavaged with ARB25 phage. Fold change was calculated as live phage versus heat-killed phage treated samples with n=3 biological replicates. Once cells reached an optical density corresponding to mid-log phase growth (absorbance between 0.4-0.5), RNA was isolated and rRNA depleted. Samples were multiplexed for sequencing on the Illumina HiSeq platform at the University of Michigan Sequencing Core. Data was analyzed using Arraystar software (DNASTAR, Inc.) using DEseq2 normalization with default parameters. Genes with significant up- or down-regulation were determined by the following criteria: genes with an average fold-change >5-fold and with at least 2/3 biological replicates with a normalized expression level >1% of the overall average, and a p-value < 0.05 (t test with Benjamini-Hochberg correction) Results: Specific capsule expression was increased in wild-type B. thetaiotaomicron during phage infection in vitro and in vivo. Many corresponding in vivo genes were upregulated as well as other surface layer proteins.

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Glycan effect on Saccharolytic Human Distal Gut Symbiont, B. thetaiotaomicron

Project description:This SuperSeries is composed of the following subset Series: GSE11944: Mucosal Glycan Foraging Enhances the Fitness and Transmission of a Saccharolytic Human Distal Gut Symbiont GSE11953: Mucosal Glycan Foraging Enhances the Fitness and Transmission of a Saccharolytic Human Distal Gut Symbiont: ECF mutant GSE11962: Growth of B. thetaiotaomicron on purified host mucosal glycans and glycan fragments Refer to individual Series

2008-11-20 | E-GEOD-11980 | biostudies-arrayexpress

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Project description:Dominant member of mammalian intestinal microflora

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Project description:Bacteroides thetaiotaomicron VPI-5482 Raw sequence reads

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Bacteroides thetaiotaomicron VPI-5482

Project description:Infection leaves a genetic and functional mark on the gut population of a commensal bacterium