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Effect of oxygen and carbon dioxide on gene expression in Bordetella bronchiseptica strain RB50

ABSTRACT: Effect of oxygen and carbon dioxide on gene expression in Bordetella bronchiseptica strain RB50

PROVIDER: PRJNA1117876 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29219600_1.fastq.gz	Fastqsanger.gz
	SRR29219600_2.fastq.gz	Fastqsanger.gz
	SRR29219601_1.fastq.gz	Fastqsanger.gz
	SRR29219601_2.fastq.gz	Fastqsanger.gz
	SRR29219602_1.fastq.gz	Fastqsanger.gz

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Effect of oxygen and carbon dioxide on gene expression in Bordetella bronchiseptica strain RB50

Project description:The classical bordetellae (Bordetella pertussis, B. parapertussis, and B. bronchiseptica) are obligate aerobes that use only oxygen as their terminal electron acceptor for electron transport-coupled oxidative phosphorylation. Therefore, access to oxygen is critical for these bacteria to survive. To better understand how B. bronchiseptica changes its gene regulation when faced with different levels of oxygen, we grew liquid cultures of B. bronchiseptica RB50 in ambient air, 5% oxygen, and 2% oxygen. We also measured how the presence of 5% carbon dioxide affected gene expression in these bacteria, since they are respiratory pathogens and therefore get exposed to higher carbon dioxide levels during infection than are found in ambient air.

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Bordetella bronchiseptica RB50

Project description:Bordetella bronchiseptica RB50 Raw sequence reads

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Bordetella bronchiseptica RB50 iron limitation 2

Project description:Bordetella bronchiseptica RB50 was shifted from iron replete to either iron depleted or iron replete media, and samples were taken post shift for transcriptional profiling

2011-09-08 | E-MEXP-3076 | biostudies-arrayexpress

Transcription profiling by array of Bordetella bronchiseptica strains RB50 and 761 grown in normal or high carbon dioxide conditions

Project description:Comparison of B. bronchiseptica strains RB50 and 761 grown in either atmospheric concentrations of oxygen and carbon dioxide (normal conditions) or in atmospheric levels of oxygen with the addition of 5% carbon dioxide into a sealed incubator (5% CO2 conditions).

2010-10-01 | E-MEXP-2875 | biostudies-arrayexpress

Bordetella bronchiseptica RB50

Project description:Bordetella bronchiseptica RB50 Transcriptome or Gene expression

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Bordetella bronchiseptica RB50 iron limitation

Project description:B. bronchiseptica RB50 was grown in medium either lacking or containing iron (ferrous sulfate). At various time points, samples were taken for gene expression analysis.

2011-12-01 | E-MEXP-3060 | biostudies-arrayexpress

Transcription profiling of Bordetella bronchiseptica strains 253 and RB50 to investigate adenylate cyclase toxin (ACT) expression

Project description:Bordetella bronchiseptica is a gram-negative respiratory pathogen that causes a diverse spectrum of respiratory disease in a wide-range of hosts. We sought to determine if strains of B. bronchiseptica differed in virulence using the mouse model of infection. Mean lethal doses (LD50) of different B. bronchiseptica strains varied widely in the murine model. B. bronchiseptica strain 253 had a LD50 that was 10-fold lower than the prototypical and fully sequenced B. bronchiseptica strain RB50. Using whole genomic transcriptome analysis covering 100% of B. bronchisetpctica strain RB50ÃÂÃÂs predicted open reading frames (ORFs), 253 was identified as lacking expression of adenylate cyclase toxin (ACT).

2007-10-01 | E-MEXP-1203 | biostudies-arrayexpress

Comparative genomic hybridization of Bordetella bronchiseptica strains RB50 and 1289

Project description:B. bronchiseptica strains RB50 and 1289 strains were grown in SS broth at 37°C with shaking overnight and genomic DNA was isolated from bacterial cultures using a DNA extraction kit (Qiagen, Valencia, CA) and digested with DpnII. For each labeling reaction, 2 ug of digested genomic DNA was randomly primed using Cy-5 and Cy-3 dye-labeled nucleotides, with BioPrime DNA labeling kits (Invitrogen, Carlsbad, CA) and the two differentially labeled reactions to be compared were combined and hybridized to a B. bronchiseptica RB50 specific long-oligonucleotide microarray.

2008-12-31 | E-MEXP-1737 | biostudies-arrayexpress

Comparative genomic hybridization of Bordetella bronchiseptica strains 253 and RB50 to investigate the cya adenylate cyclase toxin (ACT) operon

Project description:Bordetella bronchiseptica is a gram-negative respiratory pathogen that causes a diverse spectrum of respiratory disease in a wide-range of hosts. We sought to determine if strains of B. bronchiseptica differed in virulence using the mouse model of infection. Mean lethal doses (LD50) of different B. bronchiseptica strains varied widely in the murine model. B. bronchiseptica strain 253 had a LD50 that was 10-fold lower than the prototypical and fully sequenced B. bronchiseptica strain RB50. Using whole genomic transcriptome analysis covering 100% of B. bronchisetpctica strain RB50ÃÂs predicted open reading frames (ORFs), 253 was identified as lacking expression of adenylate cyclase toxin (ACT). Using whole genomic comparative genomic hybridization analysis and whole genome sequencing, we determined that the cya operon, which is required for ACT production, was absent from the 253 genome.

2007-10-01 | E-MEXP-1205 | biostudies-arrayexpress

Transcription profiling of Bordetella bronchiseptica RB50 nicotinic acid dose curve

Project description:B. bronchiseptica RB50 and a constitutive Bvg+ phase-locked mutant were grown at 37 degrees C in media containing a range of nicotinic acid concentrations.

2006-03-31 | E-TABM-28 | biostudies-arrayexpress

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