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Development of a Cellular Assay as a Personalized Model for Testing Chronic Wound Therapeutics (NS18)

ABSTRACT: Development of a Cellular Assay as a Personalized Model for Testing Chronic Wound Therapeutics (NS18)

PROVIDER: PRJNA1117953 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29225388.fastq.gz	Fastqsanger.gz
	SRR29225389.fastq.gz	Fastqsanger.gz
	SRR29225390.fastq.gz	Fastqsanger.gz
	SRR29225391.fastq.gz	Fastqsanger.gz
	SRR29225392.fastq.gz	Fastqsanger.gz

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Development of a Cellular Assay as a Personalized Model for Testing Chronic Wound Therapeutics

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Development of a Cellular Assay as a Personalized Model for Testing Chronic Wound Therapeutics (NS18)

Project description:Exudates of non-healing wounds contain drivers of pathogenicity. We utilized >800 exudates from non-healing and healing wounds of diverse etiologies, collected by three different methods, to develop a wound-specific, cell-based functional biomarker assay. Human dermal fibroblast proliferation served as readout to a) to differentiate between healing and non-healing wounds, b) follow the healing process of individual patients, and c) assess the effects of therapeutics for chronic wounds ex vivo. We observed a strong correlation between wound chronicity and inhibitory effects of individual exudates on fibroblast proliferation, with good diagnostic sensitivity (76-90%, depending on the sample collection method). Transition of a clinically non-healing to a healing phenotype restored fibroblast proliferation and extracellular matrix formation while reducing inflammatory cytokine production. Transcriptional analysis of fibroblasts exposed to ex vivo non-healing wound exudates revealed an induction of inflammatory cytokine- and chemokine pathways and the unfolded protein response, indicating that these changes may contribute to the pathology of non-healing wounds. Testing the wound therapeutics platelet derived growth factor and silver sulfadiazine yielded responses in line with clinical experience and indicate the usefulness of the assay to search for and profile new therapeutics.

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Development of a Cellular Assay as a Personalized Model for Testing Chronic Wound Therapeutics (NS79)

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Project description:Development of a Cellular Assay as a Personalized Model for Testing Chronic Wound Therapeutics (NS79)

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Development of a Cellular Assay as a Personalized Model for Testing Chronic Wound Therapeutics

Project description:This SuperSeries is composed of the SubSeries listed below: GSE268638 GSE268639

2024-05-30 | GSE268641 | GEO

Bioactive insulin-loaded electrospun wound dressings for localized drug delivery and stimulation of protein expression associated with wound healing

Project description:Effective therapy of wounds is difficult, especially for chronic, non-healing wounds, and novel therapeutics are urgently needed. This challenge can be addressed with bioactive wound dressings providing a microenvironment and facilitating cell proliferation and migration, ideally incorporating actives which initiate and/or progress effective healing upon release. In this context, electrospun scaffolds loaded with growth factors emerged as promising wound dressings due to their biocompatibility, similarity to the extracellular matrix and potential for controlled drug release. In this study, electrospun core-shell fibers were designed composed of a combination of polycaprolactone and polyethylene oxide. Insulin, a proteohormon with growth factor characteristics, was successfully incorporated into the core and was released in a controlled manner. The fibers exhibited favorable mechanical properties and a surface guiding cell migration for wound closure in combination with a high uptake capacity for wound exudate. Biocompatibility and significant wound healing effects were shown in interaction studies with human skin cells. As a new approach, analysis of the wound proteome in treated ex vivo human skin wounds clearly demonstrated a remarkable increase in wound healing biomarkers. Based on these findings, insulin-loaded electrospun wound dressings bear a high potential as effective wound healing therapeutics overcoming current challenges in the clinics.

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