Project description:Transcripitonal profiling of Escherichia coli K-12 comparing luxS mutant LW12 with wild type W3110 One-condition experiment, luxS mutant LW12 vs. wild type W3110

2010-11-11 | E-GEOD-20606 | biostudies-arrayexpress

Escherichia coli str. K-12 substr. W3110 isolate:ZK126

Project description:Escherichia coli str. K-12 substr. W3110 isolate:ZK126 Genome sequencing

| PRJNA352084 | ENA

Comparison of gene expression profiles of E. coli W3110 normal colonies vs. L-form colonies

Project description:Expression data comparing typical E.coli colonies grown on BHI and BHI+Sucrose+MgSO4 to L-form colonies grown on BHI+Sucrose+MgSO4+Penicillin G. Keywords: one time point DNA microarray analysis of E.coli colonies grown on BHI and BHI+Sucrose+MgSO4 (controls) with L-form colonies grown on BHI+Sucrose+MgSO4+Penicillin G (experimental). An isolated colony of fresh W3110 was grown for 5hrs to log phase, dilutions of the inoculmn were spread onto three media: BHI, BHI+Sucrose+MgSO4, and BHI+Sucrose+MgSO4+Penicillin G to yield isolated colonies. Isolated control colonies which grow overnight were harvested the next day while isolated L-form colonies which do not grow until 48-72 hrs were harvested after 72hrs. All colonies were harvest with a PBS moistened sterile swab and dipped into a sterile microcentrifuge tube containing 500ul of PBS. RNA was isolated from pelleted cells using MasterPure RNA Purification Kit and reverse transcribed for making probes for array hybridization on E.coli Genome 2.0 Affymetrix microarrays.

2010-02-21 | E-GEOD-14796 | biostudies-arrayexpress

Escherichia coli K-12: luxS mutant LW12 vs. wild type W3110

Project description:Transcripitonal profiling of Escherichia coli K-12 comparing luxS mutant LW12 with wild type W3110

2010-11-11 | GSE20606 | GEO

Escherichia coli K-12: luxS mutant LW12 vs. wild type W3110 under H2O2 stress

Project description:Transcriptional profiling of Escherichia coli K-12 comparing luxS mutant LW12 with wild type W3110 exposure to 10mM or 30mM hydrogen peroxide. Two-condition experiment, luxS mutant LW12 vs. wild type W3110, treatment with 10mM hydrogen peroxide for 30min or treatment with 30mM hydrogen peroxide for 30min. Two biological replicates.

2014-05-08 | E-GEOD-16335 | biostudies-arrayexpress

Escherichia coli K-12: luxS mutant LW12 vs. wild type W3110 under H2O2 stress

Project description:Transcriptional profiling of Escherichia coli K-12 comparing luxS mutant LW12 with wild type W3110 exposure to 10mM or 30mM hydrogen peroxide.

2014-05-08 | GSE16335 | GEO

RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655

Project description:Expression profiles of wild-type and SgrR mutant E. coli strains under aMG and 2-DG-induced stress. Expression profiles of E. coli overexpressing SgrS sRNA.

2013-03-26 | GSE45443 | GEO

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