Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

PI3Kg in B cells promotes antibody responses and generation of antibody-secreting cells [scRNA-seq]

ABSTRACT: PI3Kg in B cells promotes antibody responses and generation of antibody-secreting cells [scRNA-seq]

PROVIDER: PRJNA1120924 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

PI3Kg in B cells promotes antibody responses and generation of antibody-secreting cells [scRNA-seq]

Project description:The differentiation of naïve and memory B cells into antibody-secreting cells (ASCs) is a key feature of adaptive immunity. The requirement for phosphoinositide 3-kinase delta (PI3Kδ) to support B cell biology has been investigated intensively; however, specific functions of the related phosphoinositide 3-kinase-gamma (PI3Kγ) complex in B-lineage cells have not. Here we report that PI3Kγ promotes robust antibody responses induced by T cell-dependent antigens. The inborn error of immunity caused by human deficiency in PI3Kγ results in broad humoral defects, prompting our investigation of roles for this kinase in antibody responses using mouse models with bulk and single-cell transcriptome as well as B cell receptor repertoire sequencing.

2024-06-26 | GSE269300 | GEO

PI3Kg in B cells promotes antibody responses and generation of antibody-secreting cells [RNA-seq]

Project description:PI3Kg in B cells promotes antibody responses and generation of antibody-secreting cells [RNA-seq]

| PRJNA1120931 | ENA

PI3Kg in B cells promotes antibody responses and generation of antibody-secreting cells [RNA-seq]

2024-06-26 | GSE269303 | GEO

Characterization of gene expression in antibody secreting cells

Project description:Characterization of gene expression in antibody secreting cells

| PRJNA421015 | ENA

Targeting BMI-1 to deplete antibody-secreting cells in autoimmunity

Project description:We measured genes expression differences in antibody secreting cells from LynKO mice in following treatment with a BMI-1 inhibitor (PTC-028) or a vehicle control. BMI-1 inhibition lead to a reduction in antibody secreting cells in LynKO mice and in humans donors. We performed RNA sequencing to understand the impact of BMI-1 inhibition on antibody secreting cells in LynKO mice.

2023-10-05 | GSE239515 | GEO

Characterization of gene expression in antibody secreting cells

Project description:The CD19 positive antibody secreting cells (ASC) in both bone marrow (BM) have the capacity to provide immune memory in addition to cells traditionally considered long-lived, the CD19-negative BM ASC. We performed flow cytometry (FCM) immunophenotyping, fluorescence-activated cell sorting (FACS) for cell subset isolation, ELISpot assays detecting the isotype of antibody secretion as well as antibodies against vaccine derived antigens, and comparative gene expression analyses of CD19- ASC, CD19+ ASC, CD20- B cells, and CD20+ B cells from BM. The findings may aid in the understanding of the differential cell subsets created through vaccination and lead to improved vaccine strategies and production. FACS sorted tissue B cells and antibody secreting cell subset gene expression.

2018-12-03 | GSE107683 | GEO

Targeting BMI-1 to deplete antibody-secreting cells in autoimmunity

Project description:Targeting BMI-1 to deplete antibody-secreting cells in autoimmunity

| PRJNA999632 | ENA

Whole Transcriptome RNASeq Data for Cell-Sorted Antibody Secreting Cells (ASC)

Project description:We report whole transcriptome RNASeq data for cell-sorted pop2, pop3, and pop5 which are antibody-secreting cells from human peripheral blood

2018-07-12 | GSE116971 | GEO

RNA splicing in the transition from B cells to antibody secreting cells

Project description:RNA splicing in the transition from B cells to antibody secreting cells

| PRJNA450826 | ENA

High-throughput profiling of antigen-specific antibody secreting cells using an antibody secretion trap (TRAPnSeq)

Project description:High-throughput profiling of antigen-specific antibody secreting cells using an antibody secretion trap (TRAPnSeq)

| PRJNA976400 | ENA