Gallus gallus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1121483 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR29383072.fastq.gz | Fastqsanger.gz | |||
| SRR29383073.fastq.gz | Fastqsanger.gz |
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Project description:Necrotic enteritis (NE) in broiler chickens, caused by the overgrowth of toxin-producing strains of Clostridium (C.) perfringens, results in the development of necrotic lesions, compromised intestinal health, and significant economic losses in poultry production. This study aims to analyze the blood proteome of broiler chickens affected by NE, providing insights into the host's response to the infection. Using MS/MS-based proteomics, blood plasma samples from broilers with necrotic lesions of different severity were analyzed and compared to healthy controls. A total of 412 proteins were identified, with 63 showing significant differences and (for some of those) correlating with disease severity. Gene Set Enrichment Analysis (GSEA) revealed that proteins affected by NE were predominantly associated with the immune and signaling processes and extracellular matrix (ECM) structures. Notably, regulated proteins were significantly involved in bioprocesses related to complement activation, acute phase reaction, proteolysis and humoral immune response. The findings suggest that the changes in plasma proteins in response to NE are driven by the host's intensified efforts to counteract the infection, demonstrating a.o. a notable reduction in peptides from ECM-related proteins in the blood of NE-affected birds. Overall, proteomics results underscored the attempts of the host to manage tissue damage and inflammation, indicating a coordinated effort to mitigate the pathogenic impact of C. perfringens. This study provides a deeper understanding of the host-pathogen interactions and potential targets for therapeutic intervention.
2025-05-07 | PXD054172 | Pride
Project description:Gene expression profiling of clostridium perfringens infection in broilers on medicated and non-medicated diets using chicken 44k agilent microarray. To elucidate molecular and ceelular mechanisms of bacitracin effect on CP infection in chickens by microarray technology.
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Project description:Purpose: Analyze gene expression of necrotic enteritis C. perfringens in intestinal chicken loops comparing with in vitro conditions
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Project description:Necrotic enteritis is an economically important disease in poultry production caused by the opportunistic pathogen Clostridium perfringens. With the phasing out of antibiotics for prophylaxis and growth promotion, alternative solutions are needed to prevent diseases. We investigated the effect of two potentially beneficial bacteria, Lactobacillus acidophilus AG01 and Bifidobacterium animalis subspecies lactis AG02, on C. perfringens pathogenicity and observed two distinct but complementary modes of action. Live L. acidophilus AG01 cells had a strong deleterious effect on the survival of C. perfringens 25037-CP01 when grown in co-cultures, likely caused by the production of lactate resulting in the acidification of the media. Using our recently developed single cell RNA-seq method for bacteria, ProBac-seq, we observed a strong effect of the cell-free supernatant (CFS) of both bacteria on overall gene expression and the expression of virulence factors in C. perfringens 25037-CP01. Cells exposed to 5% CFS of B. animalis AG02 were enriched in RNA polymerases, and genes involved in oxidative phosphorylation and fatty acid biosynthesis. Cells exposed to 5% B. animalis AG02 or 3% L. acidophilus CFS showed significant expression reduction of netB, the virulence factor associated with necrotic enteritis. Five other virulence factors had significantly reduced expression in cells exposed to 5% B. animalis AG02 CFS. Epithelial HT-29 cells suffered significantly lower cytotoxicity when exposed to C. perfringens 25037-CP01 cells grown during exposure to 5% B. animalis AG02 CFS. These findings show that interactions among bacteria can reduce pathogenicity of an opportunistic pathogen. The two bacteria tested here reduce C. perfringens pathogenicity in different ways and could be used as an alternative to the administration of preventative antibiotics.
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Project description:Genome-wide transcriptional changes in the Clostridium perfringens-afflicted chicken intestine in an avian necrotic enteritis (NE) disease model
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Project description:Clostridium perfringens encodes at least two different quorum sensing (QS) systems, the Agr-like and LuxS, and recent studies have highlighted their importance in the regulation of toxin production and virulence. The role of QS in the pathogenesis of necrotic enteritis (NE) in poultry and the regulation of NetB, the key toxin involved, has not yet been investigated. We have generated isogenic agrB-null and complemented strains from parent CP1, and demonstrated that the virulence of the agrB-null mutant was strongly attenuated in a chicken NE model system, and restored by complementation. The production of NetB, a key NE-associated toxin, was dramatically reduced in the agrB mutant at both the transcriptional and protein level, though not in a luxS mutant. Transwell assays confirmed that the Agr-like QS system controls NetB production through a diffusible signal. Global gene expression analysis of the agrB mutant identified additional genes modulated by Agr-like QS, including operons related to phospholipid metabolism and adherence, which may also play a role in NE pathogenesis. This study provides the first evidence that the Agr-like QS system is critical for NE pathogenesis, and identifies a number of Agr-regulated genes, most notably the NetB toxin, that are potentially involved in mediating its effects. The Agr-like QS system thus may serve as a target for developing novel interventions to prevent NE in chickens.
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Project description:Purpose: Analyze gene expression during C. perfringens colonization in the chicken Transcriptomic profile of mRNA from C. perfrinegns from in vivo and in vitro conditions were determined in biological duplicates by RNA-Seq using Illumina HiSeq 2500 Comparison of gene expression through RNA sequencing of necrotic enteritis C. perfrinegns type A of in vivo (chicken loops) and in vitro (lab culture)
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