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Myelin regulatory factor (Myrf) is a critical early regulator of retinal pigment epithelial development [P0 scRNA-seq]

ABSTRACT: Myelin regulatory factor (Myrf) is a critical early regulator of retinal pigment epithelial development [P0 scRNA-seq]

PROVIDER: PRJNA1122598 | ENA |

REPOSITORIES: ENA

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Myelin regulatory factor (Myrf) is a critical early regulator of retinal pigment epithelial development [P0 scRNA-seq]

Project description:Myelin regulatory factor (Myrf) is a critical transcription factor in early retinal and retinal pigment epithelial development, and human variants in MYRF are a cause for nanophthalmos. Single cell RNA sequencing (scRNAseq) was performed on Myrf conditional knockout mice (Rx>Cre Myrffl/fl) at 3 developmental timepoints. Myrf was expressed specifically in the RPE, and expression was abrogated in Rx>Cre Myrffl/fl eyes. scRNAseq analysis revealed a loss of RPE cells at all timepoints resulting from cell death. GO-term analysis in the RPE revealed downregulation of melanogenesis and anatomic structure morphogenesis pathways, which were supported by electron microscopy and histologic analysis. Novel structural target genes including Ermn and Upk3b, along with macular degeneration and inherited retinal disease genes were identified as downregulated, and a strong upregulation of TGFß/BMP signaling and effectors was observed. Regulon analysis placed Myrf downstream of Pax6 and Mitf and upstream of Sox10 in RPE differentiation. Together, these results suggest a strong role for Myrf in the RPE maturation by regulating melanogenesis, cell survival, and cell structure, in part acting through suppression of TGFß signaling and activation of Sox10.

2025-01-01 | GSE269599 | GEO

Myelin regulatory factor (Myrf) is a critical early regulator of retinal pigment epithelial development [e15 scRNA-seq]

2025-01-01 | GSE269598 | GEO

Myelin regulatory factor (Myrf) is a critical early regulator of retinal pigment epithelial development [e13 scRNA-seq]

2025-01-01 | GSE269597 | GEO

Myelin regulatory factor (Myrf) is a critical early regulator of retinal pigment epithelial development [e15 scRNA-seq]

Project description:Myelin regulatory factor (Myrf) is a critical early regulator of retinal pigment epithelial development [e15 scRNA-seq]

| PRJNA1122599 | ENA

Myelin regulatory factor (Myrf) is a critical early regulator of retinal pigment epithelial development [e13 scRNA-seq]

Project description:Myelin regulatory factor (Myrf) is a critical early regulator of retinal pigment epithelial development [e13 scRNA-seq]

| PRJNA1122597 | ENA

Pancreatic cancer cells require the transcription factor MYRF (Myelin Regulatory Factor) to maintain ER homeostasis (ChIP-Seq).

Project description:Many tumors of endodermal origin are composed of highly secretory cancer cells that must adapt endoplasmic reticulum (ER) activity to enable proper folding of secretory proteins, prevent ER stress and thus maintain their viability. In pancreatic ductal adenocarcinoma (PDAC), well-differentiated and secretory cancer cells coexist with poorly differentiated cells with quasi-mesenchymal features. We found that the differentiated PDAC cells, but not the undifferentiated ones, expressed an ER membrane-associated transcription factor (TF), Myelin Regulatory Factor (MYRF), that is released by self-cleavage and translocates to the nucleus. MYRF expression was directly controlled by HNF1B, a TF maintaining epithelial identity in PDAC, and it acted to fine-tune the expression of genes encoding highly glycosylated and cysteine-rich secretory proteins, thus preventing ER overload. MYRF-deficient PDAC cells showed anatomical and biochemical signs of ER stress, impaired proliferation and inability to form spheroids in vitro, while in vivo they generated highly secretory but poorly proliferating and hypo-cellular tumors. These data indicate a role of MYRF in the control of homeostasis and proliferation of cancer cells with high secretory activity

2020-09-18 | GSE145627 | GEO

Pancreatic cancer cells require the transcription factor MYRF (Myelin Regulatory Factor) to maintain ER homeostasis (RNA-Seq).

2020-09-18 | GSE145628 | GEO

RNA Sequencing Analysis of Wild Type and Myrf +/− mice Retina

Project description:We have created a Myrf knockdown (Myrf +/−) mutant mouse strain and investigated the molecular phenotype of reduced MYRF expression in the retina using RNA-sequencing. As an initial finding, photoreceptor defects with impaired functions of spatial vision and retinal electrophysiology were found in Myrf +/− mice, indicating an important role of MYRF in retinal development.It was revealed through RNA-seq that the expression of phototransduction and estrogen signaling pathways reduced in Myrf +/− mice. Thus, alterations of phototransduction and estrogen signaling pathways seem to play important roles in connecting Myrf deficiency with retinal photoreceptor defects.

2020-09-12 | GSE157806 | GEO

Pancreatic cancer cells require the transcription factor MYRF (Myelin Regulatory Factor) to maintain ER homeostasis

Project description:Pancreatic cancer cells require the transcription factor MYRF (Myelin Regulatory Factor) to maintain ER homeostasis

| PRJNA607766 | ENA

SHEF1.3-derived retinal pigment epithelium for cell therapy is free of stem cell contaminants

Project description:Safety is the principle consideration with any clinical program, for which hESC and their derived products hold specific challenges. Differentiated cell products derived from hESC must be free from pluripotent cells as these could potentially form teratomas. One relevant clinical program is transplantation of retinal pigment epithelial cells (RPE) derived from hESC. This has potential for halting visual decline in conditions where the RPE layer is damaged such as age-related macular degeneration (AMD). In this study we show that whole genome gene expression analysis of SHEF1.3 starting material and the P0 pigmented RPE foci shows that the two cell types are distinct.

2016-07-03 | E-MTAB-4154 | biostudies-arrayexpress

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