Project description:Overall design Hi-C experiments were performed on untreated wild type cells at stationary and drug-treated cells (Novobiocin) of Mycoplasma Pneumoniae MPN129. We studied the chromosome organization of the genome-reduced bacterium, Mycoplasma pneumoniae, which has minimal genetic components and lacks several structural DNA-binding proteins. Platforms : Illumina HiSeq 2000 (Mycoplasma Pneumoniae MPN129)
Project description:To unravel distinct pattern of metagenomic surveillance and respiratory microbiota between Mycoplasma pneumoniae (M. pneumoniae) P1-1 and P1-2 and explore the impact of COVID-19 pandemic on epidemiological features
Project description:This dataset was used to assess the random insertion by tranposases of lox sites in Mycoplasma pneumoniae. This is part of the protocol LoxTnSeq, a new methodology to generate and catalogue libraries of genome reduction mutants. LoxTnSeq combines random integration of Lox sites by transposon mutagenesis, and the generation of mutants via cre recombinase, catalogued via deep-sequencing. When LoxTnSeq was applied to the naturally genome reduced bacterium Mycoplasma pneumoniae, we obtained a mutant pool containing 285 unique deletions. These deletions spanned from >50 bp to 28 Kb, which represent 21% of the total genome. LoxTnSeq also highlighted large regions of non-essential genes that could be removed simultaneously, and other similar regions that could not, providing a guide for future genome reductions.
Project description:To gain insight into ncRNAs functionality in bacteria, we studied the correlation of gene expression from Mycoplasma pneumoniae's ncRNAs with their overlapping ORFs over 10 different time points. This experiment contains RNAseq pair-end data from this 10 time points along Mycoplasma pneumoniae growth cycle.
Project description:We developed a serum and animal component-free medium capable of supporting robust growth of the genome-reduced bacterium Mycoplasma pneumoniae. Here, we performed RNA-seq expression analysis of the wild-type strain grown in serum-free vB13 medium.