Project description:RNA sequencing was applied to compare the transcriptome profiles of conidium, appressoruim, infected apple leaf (IL), and cellophane infectious hyphae (CIH)
Project description:A novel approach using cellophane sheets was designed to promote Pseudomonas aeruginosa twitching motility, as well as to provide convenience for cell harvest. The AmrZ regulon was defined under this specific growth condition by microarray analysis.
Project description:Full transcriptomes of the Botrytis cinerea wild-type strain B0510 and the null-mutants deltaBcVEL1 and deltaBcLAE1, cultured onto solid grape juice medium with cellophane overlays , were compared to identify BcVEL1 or/and BcLAE1-dependent genes.
Project description:Full transcriptomes of the Botrytis cinerea wild-type strain B0510, cultured in liquid medium or on cellophane sheets placed on PDB 1/4-agar plates, were compared to identify genes differentially expressed when infection cushions are formed from vegetative mycelium.
Project description:Full transcriptomes of the Botrytis cinerea wild-type strain B0510 and the null-mutants deltaBcVEL1 and deltaBcLAE1, cultured onto solid grape juice medium with cellophane overlays , were compared to identify BcVEL1 or/and BcLAE1-dependent genes. The Botrytis cinerea wild-type strain and the null-mutants deltaBcVEL1 and BcLAE1 were cultured for 48h onto solid grape juice medium with cellophane overlays. 4 replicates were performed. The 12 total-RNA samples (3 strains* 4 replicates) were used for hybridization on NimbleGen 4plex gene expression arrays (20,885 gene models from Botrytis cinerea with three 60-mer probes per gene).
