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Escherichia coli strain:K-12 MG1655

ABSTRACT: Escherichia coli RNA-Seq

PROVIDER: PRJNA1126438 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29542535_1.fastq.gz	Fastqsanger.gz
	SRR29542535_2.fastq.gz	Fastqsanger.gz
	SRR29542536_1.fastq.gz	Fastqsanger.gz
	SRR29542536_2.fastq.gz	Fastqsanger.gz
	SRR29542537_1.fastq.gz	Fastqsanger.gz

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RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens

Project description:Here, we investigated the impact of Stx2 phage carriage on Escherichia coli (E. coli) K-12 MG1655 host gene expression. Using quantitative RNA-seq analysis, we compared the transcriptome of naïve MG1655 and the lysogens carrying the Stx2 phage of the 2011 E. coli O104:H4 outbreak strain or of the E. coli O157:H7 strain PA8, which share high degree of sequence similarity.

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Escherichia coli str. K-12 substr. MG1655

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Expression analysis of Escherichia coli MG1655 K-12 WT and M-bM-^HM-^FarcA mutant

Project description:Investigation of whole genome gene expression level changes in a Escherichia coli MG1655 K-12 M-bM-^HM-^FarcA mutant, compared to the wild-type strain. The mutations engineered into this strain produce a strain lacking the ArcA protein. The results are further described in the manuscript The response regulator ArcA uses a diverse binding site architechture to globally regulate carbon oxidation in E. coli A six chip study using total RNA recovered from three separate cultures of Escherichia coli MG1655 K-12 WT and three separate cultures of the M-bM-^HM-^FarcA mutant strain. Each chip measures the expression level of 4,661 genes from Escherichia coli MG1655 K-12 with eight 60-mer probes per gene, with each probe represented twice on the array.

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Escherichia coli strain:K-12 MG1655

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Escherichia coli strain:K-12 MG1655

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