Transcriptomic Differences between Oral and Skin Keratinocytes
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1128674 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR29583239_1.fastq.gz | Fastqsanger.gz | |||
| SRR29583239_2.fastq.gz | Fastqsanger.gz | |||
| SRR29583242_1.fastq.gz | Fastqsanger.gz | |||
| SRR29583242_2.fastq.gz | Fastqsanger.gz | |||
| SRR29583245_1.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 16 |
Similar Datasets
Project description:The morphology and the behavior of skin and oral tissue keratinocytes are different. One significant dissimilarity between the two sites is the response to injury. Oral and skin keratinocytes have intrinsic differences in the response to injury and such differences are reflected in gene expression profiles. We used microarrays to investigate differences in global gene expression patterns between baseline skin and oral epithelium sheets without their underlying connective tissue.
2014-06-15 | GSE56135 | GEO
Project description:The morphology and the behavior of skin and oral tissue keratinocytes are different. One significant dissimilarity between the two sites is the response to injury. Oral and skin keratinocytes have intrinsic differences in the response to injury and such differences are reflected in gene expression profiles. We used microarrays to investigate differences in global gene expression patterns between baseline skin and oral epithelium sheets without their underlying connective tissue. Paired skin and oral epithelium was separated from the dermis for RNA extraction and hybridization on Affymetrix microarrays. Skin epidermal tissues were obtained from the tail of mice and oral epidermal tissues were obtained from the hard palate. Enzymatically isolated epithelium was used for analysis.
2014-06-15 | E-GEOD-56135 | biostudies-arrayexpress
Project description:Compared to skin wounds, oral mucosal wounds heal more rapidly with less inflammation, faster re-epithelialization, and minimal scarring. One cell type that may be a differentiating factor is keratinocytes. Using immortalized skin and oral keratinocytes, HaCaT and TIGK, respectively, we found that oral keratinocytes have an enhanced migratory and proliferative capacity. To examine the transcriptomic differences that underlie the improved healing abilities of oral keratinocytes, we generated an RNA-sequencing (RNA-seq) gene expression dataset utilizing HaCaT and TIGK. Differentially expressed genes (DEGs) were identified between HaCaT and TIGK at baseline and throughout the injury response. Gene Ontology (GO) or Reactome enrichment analysis was performed to understand the biological significance of DEGs. Processes related to inflammation and migration were uniquely enriched in TIGK. Additionally, TIGK retained its enhanced migratory capacity when grown on various basement membrane (BM) and extracellular matrix (ECM) substrates. This may be due to the differential expression of matrix metalloproteinases (MMPs) and integrins between HaCaT and TIGK. We also identified genes differentially over or under-expressed in HaCaT and TIGK following injury when compared to each respective cell type’s unwounded gene expression levels. Lastly, we showed that the post-injury secretome of TIGK promotes keratinocyte migration. Our comparative analyses suggest specific transcriptomic differences between oral and skin keratinocytes at unwounded baseline and in response to injury may underlie the distinct wound healing phenotypes seen in these two tissues. This work also provides a source of HaCaT and TIGK gene expression data over healing that can be used for future analyses.
2025-04-04 | GSE270877 | GEO
Project description:Keratinocytes cover both the skin and some oral mucosa, but the morphology of each tissue and the behavior of the keratinocytes from these two sites are different. One significant dissimilarity between the two sites is the response to injury. Oral mucosal wounds heal faster and with less inflammation than equivalent cutaneous wounds. We hypothesized that oral and skin keratinocytes might have intrinsic differences at baseline as well as in the response to injury, and that such differences would be reflected in gene expression profiles.
| S-EPMC4157746 | biostudies-literature
Project description:Wound healing within the oral mucosa results in minimal scar formation compared to wounds within the skin. We have recently demonstrated distinct differences in the ageing profiles of cells (oral mucosal and patient-matched skin fibroblasts) isolated from these tissues. We hypothesize that the increased replicative potential of oral mucosal fibroblasts may confer upon them preferential wound healing capacities. Passage-matched early cultures of oral mucosal fibroblasts and skin fibroblasts demonstrated distinct gene expression profiles with a number of genes linked to wound healing/tissue repair. We analyzed the gene expression profiles of oral mucosal and patient-matched skin fibroblasts for multiple patients both prior to (0h) and (6h) following a wounding stimulus. Differences in the gene expression profiles of oral mucosal and patient-matched skin fibroblasts were anlazyed for multiple patients both prior to (0h) and (6h) following a wounding stimulus. Serum starvation and subsequent stimulation provides a model for wounding and RNA extracted at 0h and 6h following this stimulus was hybridized to Affymetrix microarrays for analysis. We sought to compare the expression profiles both between oral and normal fibroblasts, in both serum depleted and stimulated conditions and also compare differences between patients.
2011-01-01 | E-GEOD-21648 | biostudies-arrayexpress
Project description:Intrinsic differences between oral and skin keratinocytes
| PRJNA242538 | ENA
Project description:While skin and oral mucosa share many morphological similarities, oral mucosal wounds heal more rapidly than skin wounds. Epithelial cells from oral mucosa exhibit increased migratory and proliferative capacities when compared to cells from skin, suggesting that the improved repair of mucosa may involve intrinsic differences in epithelial cells. This is an exploratory experiment to define the differential microRNA expression of baseline unwounded skin and oral mucosa epithelium.
2019-09-11 | GSE125423 | GEO
Project description:This was a hypothesis generating experiment to look at miR expression in a range of oral keratinocytes, including normal, precancer and oral cancer samples. The cells were cultures as per standard protocols (duplicate cultures) before assessment on the Affy miRNA array (1.0) one normal oral keratinocyte, one precancer, and 2 cancer samples, all in duplicate
2013-11-28 | E-GEOD-52811 | biostudies-arrayexpress
Project description:This was a hypothesis generating experiment to look at miR expression in a range of oral keratinocytes, including normal, precancer and oral cancer samples. The cells were cultures as per standard protocols (duplicate cultures) before assessment on the Affy miRNA array (1.0)
2013-11-28 | GSE52811 | GEO
Project description:Miccrococcal nuclease sequencing (MNase-seq) of chromatin from control and Snail2-expressing oral keratinocytes
2017-12-31 | GSE65191 | GEO