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Effect of flg22 peptide on Arabidopsis seedling roots with endodermis-expressed FLS2 in fls2 mutant background

ABSTRACT: Effect of flg22 peptide on Arabidopsis seedling roots with endodermis-expressed FLS2 in fls2 mutant background

PROVIDER: PRJNA1129099 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29621818.fastq.gz	Fastqsanger.gz
	SRR29621819.fastq.gz	Fastqsanger.gz
	SRR29621820.fastq.gz	Fastqsanger.gz
	SRR29621821.fastq.gz	Fastqsanger.gz
	SRR29621822.fastq.gz	Fastqsanger.gz

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Effect of flg22 peptide on Arabidopsis seedling roots with endodermis-expressed FLS2 in fls2 mutant background

Project description:To compare transcriptional outputs of FLS2 and SGN3 pathways solely within the endodermis, we conducted comparative RNAseq analysis of pCASP1::FLS2 fls2 and wild-type seedling roots treated by flg22 and CIF2 respectively, at three timepoints (30, 120, 480 min) (CIF2 responses data previously published by Fujita et al 2020). The receptor mutant controls sgn3 and fls2, displayed minimal gene responses to respective peptides.

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Project description:We reported the flg22-triggered immune responses in roots affect the iron deficiency responses and may link to the function of FLS2 and IMA1 in the root. To identify the underlying mechanism of how the root transcriptome profiles respond to +Fe, +Fe+flg22, -Fe, -Fe+flg22 respectively, and if the flg22 responses is dependent on the function of FLS2 and IMA1 in the root, we performed an mRNA-seq experiments in Col-0, fls2 and UBQ10::mCitrine-IMA1 with different treatments. The differentially expressed genes in response to +Fe, +Fe+flg22, -Fe, -Fe+flg22 were analyzed. It has 36 samples in total, with 3 biogical replicates for each condition and each genotype.

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