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Biosensor-guided evolution boosts itaconic acid production, unveiling novel insights on the stringent response

ABSTRACT: Biosensor-guided evolution boosts itaconic acid production, unveiling novel insights on the stringent response

PROVIDER: PRJNA1129262 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29634527_1.fastq.gz	Fastqsanger.gz
	SRR29634527_2.fastq.gz	Fastqsanger.gz
	SRR29634528_1.fastq.gz	Fastqsanger.gz
	SRR29634528_2.fastq.gz	Fastqsanger.gz
	SRR29634529_1.fastq.gz	Fastqsanger.gz

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Biosensor-guided evolution boosts itaconic acid production, unveiling novel insights on the stringent response

Project description:Acetate is a cost-effective and sustainable carbon source that, despite its potential, remains underutilized. This study employed biosensor-assisted adaptive laboratory evolution (ALE) to enhance itaconic acid production and acetate metabolism in Escherichia coli. The evolved E. coli W strains exhibited 65% increase in itaconic acid production and 71% increase in growth rate, and 45% increase in itaconic acid yield. A common 31-kb genomic deletion was identified in the evolved strains, with two genes, ecw_m2276 and ecw_m2277, driving the observed phenotypic changes. The evolved strains exhibited an intensified stringent response, which enhanced the acetate-utilizing pathway and resulted in over a 5,000% increase in the expression of the glyoxylate shunt, thereby boosting microbial growth. Overexpression of relA further replicated these enhanced phenotypes. Our findings highlight not only significant physiological improvements but also present a novel strategy for enhancing microbial growth and bioproduction from acetate, offering valuable insights for industrial biotechnology applications.

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Project description:Zn (Ⅱ) and Fe (Ⅱ) are the essential metal elements for the growth of microorganisms. It illustrated that more itaconic acid was achieved for 0.25 g/L ZnSO4·2H2O and 0.40 g/L FeSO4·2H2O than the control with 0.15 g/L ZnSO4·2H2O and 0.16 g/L FeSO4·2H2O after single factor assays for Aspergillus terreus.We furhter carried out transcriptome assays to uncover molecular mechanism of the enhanced itaconic acid fermentability for A. terreus with metal ion.Therefore, our study would provide a reference metal ion concentration ratio for itaconic and other biochemicals production.

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