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Identification of candidate genes associated with the double flower of Brassica napus using RNA-seq

ABSTRACT: Identification of candidate genes associated with the double flower of Brassica napus using RNA-seq

PROVIDER: PRJNA1130462 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29664305_1.fastq.gz	Fastqsanger.gz
	SRR29664305_2.fastq.gz	Fastqsanger.gz
	SRR29664306_1.fastq.gz	Fastqsanger.gz
	SRR29664306_2.fastq.gz	Fastqsanger.gz
	SRR29664307_1.fastq.gz	Fastqsanger.gz

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Identification of candidate genes associated with the double flower of Brassica napus using RNA-seq

Project description:It revealed that DEGs associated with floral development, including BnaC06.ARGOS, BnaC09.BPE, BnaA03.AUX1 and BnaAP2, and BnaC05.ERS2 involved in ethylene signalling potentially contribute to the double flower trait in B. napus.

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Production of Red Flower Rapeseed by Ectopic Expression of OvPAP2 Identified in Brassica napus-Orychophragmus violaceus Addition Line by Comparative Transcriptome Analysis

Project description:Oilseed rape is both an important oleaginous crop and agriculture sightseeing crop whereas has relatively scanty flower color. As natural ﬂavonoids, Anthocyanin are responsible for the attractive red, purple, and blue colors of various tissues in higher plants, especially for the ornamental plants flower. One Brassica napus-Orychophragmus violaceus disomic addition line (M4) obtained previously exhibits red petals whichresult from anthocyanin biosynthesis. Transcriptome analysis of M4, B. napus (H3), natural individuals of O. violaceus with purple petals (OvP) and white petals (OvW) revealed that most of structural genes for the anthocyanin synthesis were up-regulated in both M4 and OvP, especially key gene ANS in the last step. Reads assembling and sequence alignment showed that the regulatory DEG PAP2 in M4 was from the transcript of O. violaceus. OvPAP2 was transformed into Arabidopsis thaliana and B. napus driven by the CaMV35S promoter and the rape petal-specific prompter XY355. Transgenic A. thaliana plants showed different levels of purple pigments in most of the organs, including the petals, and transgenic B. napus flowers exhibited restricted accumulation of anthocyanins in stamens when driven by CaMV35S promoter, but generated both red petals and anthers driven by the XY355 promoter. These results provided a platform for expounding the anthocyanin biosynthesis pathway in B. napus petals and give a successful case for flower color modification of the agriculture sightseeing rape.

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RNAseq of Brassica napus seed

Project description:mRNA expression profiling of the embryo, endosperm (micropylar, peripheral, chalazal), and seed coat (outer, inner, chalazal, chalazal proliferating tissue) of the developing Brassica napus seed. Tissues were isolated using laser microdissection (LMD) from Brassica napus seeds at the globular, heart, and mature green stages of seed development.

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Project description:Understanding the regulation of lipid metabolism is vital for genetic engineering of Brassica napus (B. napus) to increase oil yield or modify oil composition. We report the application of Illumina Hiseq 2000 for transcriptome profiling of seeds of B. napus at different developmental stages, which may uncover the dynamic changes in lipid metabolism and reveal key genes involved in lipid biosynthesis and degradation. Total RNA from developing seeds at 2, 4, 6, and 8 weeks after pollination (WAP) were isolated and sequenced separately. The gene expression levels of all samples were quantified and normalized by the DESeq normalization. We found that the biosynthesis of fatty acids is a dominant cellular process from 2 to 6 WAP, while the degradation mainly happens after 6 WAP. Two genes, encoding for acetyl-CoA carboxylase and acyl-ACP desaturase, might be critical for fatty acid biosynthesis in oil rape seeds. This study provides insight into the mechanism underlying lipid metabolism and reveals candidate genes that are worthy of further investigation for their values in genetic engineering of B. napus. Whole Transcriptome profiling of developing Brassica napus seeds at 2, 4, 6, 8 WAP by RNA sequencing using Illumina HiSeq 2000.

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